[English] 日本語
![](img/lk-miru.gif)
- PDB-1vga: Structures of unligated and inhibitor complexes of W168F mutant o... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1vga | ||||||
---|---|---|---|---|---|---|---|
Title | Structures of unligated and inhibitor complexes of W168F mutant of Triosephosphate Isomerase from Plasmodium falciparum | ||||||
![]() | Triosephosphate isomerase | ||||||
![]() | ISOMERASE / Plasmodium falciparum / Triosephosphate Isomerase / Loop6 mutant / flexibility / intermediate position | ||||||
Function / homology | ![]() triose-phosphate isomerase / triose-phosphate isomerase activity / glyceraldehyde-3-phosphate biosynthetic process / glycerol catabolic process / gluconeogenesis / glycolytic process / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Eaazhisai, K. / Balaram, H. / Balaram, P. / Murthy, M.R.N. | ||||||
![]() | ![]() Title: Structures of Unliganded and Inhibitor Complexes of W168F, a Loop6 Hinge Mutant of Plasmodium falciparum Triosephosphate Isomerase: Observation of an Intermediate Position of Loop6 Authors: Eaazhisai, K. / Balaram, H. / Balaram, P. / Murthy, M.R.N. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 191.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 155.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 440.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 456.1 KB | Display | |
Data in XML | ![]() | 39.9 KB | Display | |
Data in CIF | ![]() | 57.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1woaC ![]() 1wobC ![]() 1ydvS ![]() 1vgb ![]() 1vgd S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
| ||||||||
Details | The biological assembly is a dimer. There are two dimers in the asymmetric unit (AB and CD) |
-
Components
#1: Protein | Mass: 27958.703 Da / Num. of mol.: 4 / Mutation: W168F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.253 Å3/Da / Density % sol: 43.3 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: PEG1450, Lithium sulfate and Soduim acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→17.21 Å / Num. all: 93113 / Rmerge(I) obs: 0.036 |
Reflection shell | Resolution: 1.8→1.86 Å / Rmerge(I) obs: 0.214 / % possible all: 89 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1YDV Resolution: 1.8→17.2 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→17.2 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.01
|