+Open data
-Basic information
Entry | Database: PDB / ID: 1vb7 | ||||||
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Title | Solution structure of the PDZ domain of PDZ and LIM domain 2 | ||||||
Components | PDZ and LIM domain 2 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PDZ domain PDZ-LIM protein / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information muscle structure development / filamin binding / muscle alpha-actinin binding / cortical actin cytoskeleton / myosin heavy chain binding / alpha-actinin binding / filamentous actin / stress fiber / adherens junction / Z disc ...muscle structure development / filamin binding / muscle alpha-actinin binding / cortical actin cytoskeleton / myosin heavy chain binding / alpha-actinin binding / filamentous actin / stress fiber / adherens junction / Z disc / actin binding / heart development / actin cytoskeleton organization / metal ion binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Hatta, R. / Hayashi, F. / Yoshida, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the PDZ domain of PDZ and LIM domain 2 Authors: Hatta, R. / Hayashi, F. / Yoshida, M. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1vb7.cif.gz | 527.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1vb7.ent.gz | 442.2 KB | Display | PDB format |
PDBx/mmJSON format | 1vb7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vb/1vb7 ftp://data.pdbj.org/pub/pdb/validation_reports/vb/1vb7 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9707.788 Da / Num. of mol.: 1 / Fragment: PDZ domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Gene: cDNA 2310041E13 / Plasmid: P030203-89 / References: UniProt: Q8R1G6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.28mM 13C, 15N-labeled protein; 20mM phosphate buffer; 100mM NaCl; 1mM d10-DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120 / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |