THE FIRST THREE RESIDUES IN THE SEQRES RECORDS BELOW ARE THE REMANENTS OF A TEV CLEAVED HIS TAG AND ...THE FIRST THREE RESIDUES IN THE SEQRES RECORDS BELOW ARE THE REMANENTS OF A TEV CLEAVED HIS TAG AND DO NOT FORM PART OF THE OBSCURIN SH3 DOMAIN
-
実験情報
-
実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
3D 15N-RESOLVED NOESY-HSQC
1
2
1
3D 13C RESOLVED NOESY-HSQC
NMR実験の詳細
Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED OBSH3
-
試料調製
試料状態
イオン強度: 200 mM / pH: 5.76 / 圧: 1 atm / 温度: 298 K
-
NMR測定
NMRスペクトロメーター
タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz
-
解析
NMR software
名称
バージョン
開発者
分類
CNS
1.1
BRUNGERET.AL.
精密化
CNS/ARIA
構造決定
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: STANDARD ARIA SIMULATED ANNEALING PROTOCOL ACCORDING TO NILGES. ALL DEFAULT PARAMETERS COMING WITH VERSION 1.2 WERE USED APART FROM A NUMBER OF 200 STRUCTURES CALCULATED IN ITERATION 8 OF ...詳細: STANDARD ARIA SIMULATED ANNEALING PROTOCOL ACCORDING TO NILGES. ALL DEFAULT PARAMETERS COMING WITH VERSION 1.2 WERE USED APART FROM A NUMBER OF 200 STRUCTURES CALCULATED IN ITERATION 8 OF WHICH 20 WHERE SELECTED. NO WATER REFINEMENT WAS USED.
NMRアンサンブル
コンフォーマー選択の基準: LEAST RESTRAINT VIOLATION 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20