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Open data
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Basic information
Entry | Database: PDB / ID: 1uzb | ||||||
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Title | 1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE | ||||||
![]() | 1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE | ||||||
![]() | OXIDOREDUCTASE / DEHYDROGENASE / 1-PYRROLINE-5-CARBOXYLATE / RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE / RSGI / STRUCTURAL GENOMICS | ||||||
Function / homology | ![]() L-glutamate gamma-semialdehyde dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase activity / proline catabolic process to glutamate / cytoplasmic side of plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tahirov, T.H. / Inagaki, E. | ||||||
![]() | ![]() Title: Crystal Structure of Thermus Thermophilus Delta(1)- Pyrroline-5-Carboxylate Dehydrogenase. Authors: Inagaki, E. / Ohshima, N. / Takahashi, H. / Kuroishi, C. / Yokoyama, S. / Tahirov, T.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 239.5 KB | Display | ![]() |
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PDB format | ![]() | 193.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2bhpC ![]() 2bhqC ![]() 2bjaC ![]() 2bjkC ![]() 2iy6C ![]() 1bi9S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57112.922 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MRD / ( #3: Water | ChemComp-HOH / | Sequence details | N-TERMINAL METHIONINE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 50.03 % | ||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 5.2 Details: SITTING DROP VAPOR DIFFUSION METHOD. 1-3 DAYS. 10 MG/ML OF PROTEIN SOLUTION WAS MIXED WITH 32% V/V MPD, 0.05 M SODIUM CITRATE PH 5.2. THE SODIUM CITRATE WAS REPLACED BY SODIUM ACETATE PH 5.2 ...Details: SITTING DROP VAPOR DIFFUSION METHOD. 1-3 DAYS. 10 MG/ML OF PROTEIN SOLUTION WAS MIXED WITH 32% V/V MPD, 0.05 M SODIUM CITRATE PH 5.2. THE SODIUM CITRATE WAS REPLACED BY SODIUM ACETATE PH 5.2 BEFORE THE DATA COLLECTION | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 298 K / pH: 5.2 / Method: vapor diffusion, sitting drop / Details: Inagaki, E., (2005) Acta Cryst., F61, 609. | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU RAXIS-V / Detector: IMAGE PLATE / Date: Feb 15, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→30 Å / Num. obs: 211697 / % possible obs: 98.6 % / Observed criterion σ(I): -1 / Redundancy: 3.51 % / Biso Wilson estimate: 10.7 Å2 / Rmerge(I) obs: 0.045 / Net I/σ(I): 38.99 |
Reflection shell | Resolution: 1.4→1.42 Å / Rmerge(I) obs: 0.181 / Mean I/σ(I) obs: 7.75 / % possible all: 97.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1BI9 Resolution: 1.4→29.53 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 1254000.52 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 79.5954 Å2 / ksol: 0.446459 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.4→29.53 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.4→1.49 Å / Rfactor Rfree error: 0.005 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 30 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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