Mass: 6407.543 Da / Num. of mol.: 1 Fragment: CHIMERIC PROTEIN OF LEKTI DOMAIN ONE, RESIDUES 23-77 Mutation: YES Source method: isolated from a genetically manipulated source Details: DISULFIDE BONDS BETWEEN CYS 8 AND CYS 44, BETWEEN CYS 22 AND CYS 41 Source: (gene. exp.) HOMO SAPIENS (human) / Tissue: I.E. VAGINAL EPITHELIUM / Plasmid: PET32A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): ORIGAMI / Variant (production host): DE3 / References: UniProt: Q9NQ38
Compound details
ENGINEERED MUTATION IN CHAIN A PRO 28 FROM PHE ENGINEERED MUTATION IN CHAIN A ILE 29 FROM PHE
Sequence details
THE LEKTI SEQUENCE PROVIDED BY SWISSPROT DIFFERS FROM THAT GIVEN IN THE ORIGINAL PAPER BY MAEGERT ...THE LEKTI SEQUENCE PROVIDED BY SWISSPROT DIFFERS FROM THAT GIVEN IN THE ORIGINAL PAPER BY MAEGERT ET AL., 1999. IN THIS ENTRY THE SEQUENCE REFERS TO THE ORIGINAL PAPER AND IS ALSO IDENTICAL (EXCEPT FOR THE TWO MUTATIONS AT POSITIONS 28 AND 29) TO THAT OF LEKTI DOMAIN ONE (HF6478, PDB-CODE 1HDL) AS ISOLATED FROM HUMAN BLOOD FILTRATE (REFERENCES: MAGERT ET AL., 1999, J. BIOL. CHEM. 274, 21499-21502. LAUBER ET AL.,2001, PROTEIN EXPR. PURIF. 22, 108-112; LAUBER ET AL., 2003, J. MOL. BIOL., 328, 205-219.) THUS, THE SEQUENCE IN THIS ENTRY REFERS TO RESIDUES 23 TO 77 OF FULL-LENGTH LEKTI.
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D-TOCSY
1
2
1
2D-COSY
1
3
1
2D-NOESY
1
4
1
1H
1
5
1
15N-HSQC
1
6
1
HNHA
1
7
1
3D-1H
1
8
1
15N-TOCSY-HSQC
1
9
1
3D-1H
1
10
1
15N-NOESY-HSQC
1
11
1
3D-1H
1
12
1
15N
1
13
1
15N-HMQC-NOESY-HSQC
NMR details
Text: THIS STRUCTURE WAS DETERMINED USING STANDARD NMR-TECHNIQUES ON 15N-LABELED AND UNLABELED PROTEIN
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Sample preparation
Sample conditions
Ionic strength: 10 mM / pH: 5.0 / Pressure: 1 atm / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz
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