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Yorodumi- PDB-1uis: The 2.0 crystal structure of eqFP611, a far-red fluorescent prote... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1uis | ||||||
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Title | The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor | ||||||
Components | red fluorescent protein FP611 | ||||||
Keywords | LUMINESCENT PROTEIN | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Entacmaea quadricolor (sea anemone) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Petersen, J. / Wilmann, P.G. / Beddoe, T. / Oakley, A.J. / Devenish, R.J. / Prescott, M. / Rossjohn, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: The 2.0A crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor Authors: Petersen, J. / Wilmann, P.G. / Beddoe, T. / Oakley, A.J. / Devenish, R.J. / Prescott, M. / Rossjohn, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uis.cif.gz | 107.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uis.ent.gz | 83.4 KB | Display | PDB format |
PDBx/mmJSON format | 1uis.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ui/1uis ftp://data.pdbj.org/pub/pdb/validation_reports/ui/1uis | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 26314.053 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Entacmaea quadricolor (sea anemone) / Production host: Escherichia coli (E. coli) / References: UniProt: Q8ISF8 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.3 Details: PEG 8000, sodium acetate, calcium acetate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH3R / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jun 20, 2003 |
Radiation | Monochromator: osmic mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 140379 / Num. obs: 137625 / % possible obs: 96 % / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2→2.07 Å / % possible all: 79.7 |
Reflection | *PLUS Lowest resolution: 50 Å / Num. obs: 37625 / % possible obs: 91.2 % / Redundancy: 3.6 % / Num. measured all: 134722 / Rmerge(I) obs: 0.046 |
Reflection shell | *PLUS % possible obs: 79.7 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 2.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refinement | *PLUS Lowest resolution: 50 Å / % reflection Rfree: 4 % / Rfactor Rfree: 0.253 / Rfactor Rwork: 0.214 | ||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||
Refine LS restraints | *PLUS
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