+Open data
-Basic information
Entry | Database: PDB / ID: 1uh8 | ||||||
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Title | Crystal structure of rhizopuspepsin at pH 8.0 | ||||||
Components | hizopuspepsin I | ||||||
Keywords | HYDROLASE / pepsin / aspartic proteinase | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Rhizopus microsporus var. chinensis (fungus) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Prasad, B.V.L.S. / Suguna, K. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2003 Title: Effect of pH on the structure of rhizopuspepsin. Authors: Prasad, B.V. / Suguna, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uh8.cif.gz | 79.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uh8.ent.gz | 58.9 KB | Display | PDB format |
PDBx/mmJSON format | 1uh8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1uh8_validation.pdf.gz | 415.6 KB | Display | wwPDB validaton report |
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Full document | 1uh8_full_validation.pdf.gz | 419.2 KB | Display | |
Data in XML | 1uh8_validation.xml.gz | 16.7 KB | Display | |
Data in CIF | 1uh8_validation.cif.gz | 24.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uh/1uh8 ftp://data.pdbj.org/pub/pdb/validation_reports/uh/1uh8 | HTTPS FTP |
-Related structure data
Related structure data | 1uh7C 1uh9C 2aprS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34312.918 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Rhizopus microsporus var. chinensis (fungus) Species: Rhizopus microsporus / Strain: var. chinensis References: UniProt: Q02016, UniProt: P06026*PLUS, EC: 3.4.23.6 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.3 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: sodium cacodylate, calcium acetate, VAPOR DIFFUSION, HANGING DROP, pH 8.00, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: OSMIC MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 93785 / Num. obs: 17607 / % possible obs: 97.1 % / Rmerge(I) obs: 0.089 |
Reflection shell | Resolution: 2.3→2.34 Å / Rmerge(I) obs: 0.089 / % possible all: 97 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2APR Resolution: 2.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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