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Yorodumi- PDB-1u6b: CRYSTAL STRUCTURE OF A SELF-SPLICING GROUP I INTRON WITH BOTH EXONS -
+Open data
-Basic information
Entry | Database: PDB / ID: 1u6b | |||||||||
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Title | CRYSTAL STRUCTURE OF A SELF-SPLICING GROUP I INTRON WITH BOTH EXONS | |||||||||
Components |
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Keywords | STRUCTURAL PROTEIN/RNA / INTRON / EXON / RIBOZYME / GROUP I / U1A / RNA / STRUCTURAL PROTEIN-RNA COMPLEX | |||||||||
Function / homology | Function and homology information U1 snRNP binding / U1 snRNP / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / spliceosomal complex / mRNA splicing, via spliceosome / DNA binding / RNA binding / nucleoplasm ...U1 snRNP binding / U1 snRNP / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / spliceosomal complex / mRNA splicing, via spliceosome / DNA binding / RNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 3.1 Å | |||||||||
Authors | Adams, P.L. / Stahley, M.R. / Kosek, A.B. / Wang, J. / Strobel, S.A. | |||||||||
Citation | Journal: Nature / Year: 2004 Title: Crystal Structure of a Self-Splicing Group I Intron with Both Exons. Authors: Adams, P.L. / Stahley, M.R. / Kosek, A.B. / Wang, J. / Strobel, S.A. #1: Journal: To be published Title: Crystal Structure of a Group I Intron Splicing Intermediate Authors: Adams, P.L. / Stahely, M.R. / Gill, M.L. / Berman, C. / Kosek, A.B. / Wang, J. / Strobel, S.A. #2: Journal: To be published Title: RNA Kink Turns to the Left and to the Right Authors: Strobel, S.A. / Adams, P.L. / Stahley, M.R. / Wang, J. #3: Journal: Cell(Cambridge,Mass.) / Year: 1981 Title: In Vitro Splicing of the Ribosomal RNA Precursor of Tetrahymena: Involvement of a Guanosine Nucleotide in the Excision of Intervening Sequence Authors: Cech, T.R. / Zaug, A.J. / Grabowaski, P.J. #4: Journal: Nature / Year: 1992 Title: Self-Splicing Intron in tRNA Genes of Widely Divergent Bacteria Authors: Reinhold-Hurek, B. / Shub, D.A. | |||||||||
History |
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Remark 999 | SEQUENCE NUCLEOTIDES 1001B TO 1014B BELONG TO AN ENGINEERED U1A LOOP AND ARE INSERTED BETWEEN GUA- ...SEQUENCE NUCLEOTIDES 1001B TO 1014B BELONG TO AN ENGINEERED U1A LOOP AND ARE INSERTED BETWEEN GUA-107B AND CYT-112B. THERE ARE NO NUCLEOTIDES BETWEEN GUA-1B AND GUA-5B DUE TO A CLONING DESIGN. O2' OF DEOXY MODIFICATION OF RNA NUCLEOTIDES ARE INCLUDED WITH Q=0.00 AND B=0.00. THEY ARE AT GUA-206C, CYT-1C, CYT-205C, AND THY-1D. CHAINS B AND C ARE TWO PARTS OF THE ORIGINAL ONE INTRON SEQUENCE BECAUSE OF AN EXPERIMENTAL DESIGN. THE U1A PROTEIN USED IN THIS STUDY WAS A DOUBLE MUTANT IN WHICH TYR-31/GLN-36 WAS REPLACED WITH HIS-31/ARG-36. THE FIRST THREE RESIDUES IN U1A PROTEIN WERE MISSING IN THE STRUCTURE. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1u6b.cif.gz | 164.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1u6b.ent.gz | 120.1 KB | Display | PDB format |
PDBx/mmJSON format | 1u6b.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u6/1u6b ftp://data.pdbj.org/pub/pdb/validation_reports/u6/1u6b | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-RNA chain , 3 types, 3 molecules BCD
#1: RNA chain | Mass: 64057.023 Da / Num. of mol.: 1 / Fragment: GROUP I INTRON / Source method: obtained synthetically / Details: RNA WAS IS TRANSCRIBED BY T7 RNA POLYMERASE. |
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#2: RNA chain | Mass: 7045.354 Da / Num. of mol.: 1 / Fragment: GROUP I EXON / Source method: obtained synthetically |
#3: RNA chain | Mass: 909.623 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Protein , 1 types, 1 molecules A
#4: Protein | Mass: 11340.315 Da / Num. of mol.: 1 / Mutation: Y31H,Q36R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SNRPA / Plasmid: PET11 / Production host: Escherichia coli (E. coli) / References: UniProt: P09012 |
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-Non-polymers , 3 types, 72 molecules
#5: Chemical | ChemComp-K / #6: Chemical | ChemComp-MG / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.4 Å3/Da / Density % sol: 72.06 % |
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Crystal grow | pH: 6.8 / Details: pH 6.80 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jul 20, 2003 |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 24640 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 3.1→48.22 Å / Cor.coef. Fo:Fc: 0.881 / Cor.coef. Fo:Fc free: 0.872 / SU B: 22.65 / SU ML: 0.382 / Cross valid method: THROUGHOUT / ESU R: 1.805 / ESU R Free: 0.444 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.422 Å2
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Refinement step | Cycle: LAST / Resolution: 3.1→48.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.1→3.267 Å / Total num. of bins used: 10 /
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Refinement TLS params. | Method: refined / Origin x: 42.8243 Å / Origin y: 86.5701 Å / Origin z: 61.9647 Å
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