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- PDB-1tzn: Crystal Structure of the Anthrax Toxin Protective Antigen Heptame... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1tzn | ||||||
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Title | Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore bound to the VWA domain of CMG2, an anthrax toxin receptor | ||||||
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![]() | TOXIN RECEPTOR/TOXIN / heptamer / TOXIN RECEPTOR-TOXIN COMPLEX | ||||||
Function / homology | ![]() symbiont-mediated suppression of host MAPK cascade / collagen fibril organization / host cell cytosol / uterus development / Uptake and function of anthrax toxins / single fertilization / host cell endosome membrane / protein homooligomerization / transmembrane signaling receptor activity / toxin activity ...symbiont-mediated suppression of host MAPK cascade / collagen fibril organization / host cell cytosol / uterus development / Uptake and function of anthrax toxins / single fertilization / host cell endosome membrane / protein homooligomerization / transmembrane signaling receptor activity / toxin activity / endosome membrane / external side of plasma membrane / endoplasmic reticulum membrane / host cell plasma membrane / cell surface / extracellular region / metal ion binding / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lacy, D.B. / Wigelsworth, D.J. / Melnyk, R.A. / Collier, R.J. | ||||||
![]() | ![]() Title: Structure of heptameric protective antigen bound to an anthrax toxin receptor: A role for receptor in pH-dependent pore formation Authors: Lacy, D.B. / Wigelsworth, D.J. / Melnyk, R.A. / Harrison, S.C. / Collier, R.J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.8 MB | Display | ![]() |
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PDB format | ![]() | 1.5 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 727 KB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 400.9 KB | Display | |
Data in CIF | ![]() | 524.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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Unit cell |
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Details | The biological assembly is one heptamer |
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Components
#1: Protein | Mass: 63019.012 Da / Num. of mol.: 14 / Fragment: 63-kDa domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 19840.791 Da / Num. of mol.: 14 / Fragment: VWA domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | ChemComp-CA / #4: Chemical | ChemComp-MG / Has protein modification | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 63 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 8000, sodium chloride, Tris, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 15, 2004 / Details: null |
Radiation | Monochromator: horizontally bent Si(III) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9186 Å / Relative weight: 1 |
Reflection | Resolution: 4.3→20 Å / Num. all: 120016 / Num. obs: 120016 / % possible obs: 97.3 % / Observed criterion σ(I): 1.8 / Redundancy: 2.7 % / Rsym value: 0.165 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 4.3→4.45 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 1.8 / Num. unique all: 12124 / Rsym value: 0.449 / % possible all: 98.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3.6 heptameric prepore model Resolution: 4.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 4.3→20 Å
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Refine LS restraints |
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