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Open data
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Basic information
Entry | Database: PDB / ID: 1tyr | ||||||
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Title | TRANSTHYRETIN COMPLEX WITH RETINOIC ACID | ||||||
![]() | TRANSTHYRETIN | ||||||
![]() | RETINOL-BINDING / RETINOL-BINDING PROTEIN | ||||||
Function / homology | ![]() Retinoid cycle disease events / The canonical retinoid cycle in rods (twilight vision) / thyroid hormone binding / purine nucleobase metabolic process / Non-integrin membrane-ECM interactions / Retinoid metabolism and transport / hormone activity / azurophil granule lumen / Amyloid fiber formation / Neutrophil degranulation ...Retinoid cycle disease events / The canonical retinoid cycle in rods (twilight vision) / thyroid hormone binding / purine nucleobase metabolic process / Non-integrin membrane-ECM interactions / Retinoid metabolism and transport / hormone activity / azurophil granule lumen / Amyloid fiber formation / Neutrophil degranulation / extracellular space / extracellular exosome / extracellular region / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Zanotti, G. / D'Acunto, M.R. / Malpeli, G. / Folli, C. / Berni, R. | ||||||
![]() | ![]() Title: Crystal structure of the transthyretin--retinoic-acid complex Authors: Zanotti, G. / D'Acunto, M.R. / Malpeli, G. / Folli, C. / Berni, R. #1: ![]() Title: Crystallographic Studies on Complexes between Retinoids and Plasma Retinol-Binding Protein Authors: Zanotti, G. / Marcello, M. / Malpeli, G. / Folli, C. / Sartori, G. / Berni, R. #2: ![]() Title: Crystal Structure Determination at 2.3 Angstroms of Human Transthyretin-3',5'-Dibromo-2',4,4', 6-Tetra-Hydroxyaurone Complex Authors: Ciszak, E. / Cody, V. / Luft, J.R. #3: ![]() Title: Mechanism of Molecular Recognition. Structural Aspects of 3,3'-Diiodo-L-Thyronine Binding to Human Serum Transthyretin Authors: Wojtczak, A. / Luft, J. / Cody, V. #4: ![]() Title: Protein-DNA and Protein-Hormone Interactions in Prealbumin: A Model of the Thyroid Hormone Nuclear Receptor? Authors: Blake, C.C.F. / Oatley, S.J. #5: ![]() Title: Structure of Human Plasma Prealbumin at 2.5 Angstroms Resolution, a Preliminary Report on the Polypeptide Chain Conformation, Quaternary Structure and Thyroxine Binding Authors: Blake, C.C.F. / Geisow, M.J. / Swan, I.D.A. / Rerat, C. / Rerat, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.7 KB | Display | ![]() |
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PDB format | ![]() | 49.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 476.9 KB | Display | ![]() |
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Full document | ![]() | 498.3 KB | Display | |
Data in XML | ![]() | 11.2 KB | Display | |
Data in CIF | ![]() | 14.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | THE ENTIRE PROTEIN IS A TETRAMER, BUT THE ASYMMETRIC UNIT CONTAINS A DIMER. THE TWO DIMERS ARE RELATED BY A TWO FODL AXIS, WHILST THE TWO INDEPENDENT MONOMERS IN THE DIMER ARE RELATED BY A PSEUDO TWO-FOLD AXIS. THE LIGAND IS BOUND IN THE INTERNAL CHANNEL, MADE UP BY THE TWO INDEPENDENT MONOMERS. A CRYSTALLOGRAPHIC TWO-FOLD AXIS RUNS THROUGH THE CHANNEL, MAKING IT SYMMETRIC. MOREOVER, PERPENDICULAR TO THE PREVIOUS ONE, THERE IS THE PSEUDO TWO-FOLD, WHICH MAKES THE TWO HALFS OF THE CHANNEL NEARLY IDENTICAL. AS A RESULT OF ALL THAT, TWO INDEPENDENT BINDING SITES ARE PRESENT PER TERAMER, BUT, SINCE THE LIGAND IN THIS CASE DOES NOT PRESENT ANY SYMMETRY, EACH LIGAND IN THE CRYSTAL APPEARS TO BE SUPERIMPOSED TO ITS SYMMETRY-RELATED. IN CONCLUSION, FOUR RETINOIC ACID BOUND MOLECULES ARE SEEN, EACH PAIRS SUPERIMPOSED. IN ADDITION, SOLUTION DATA STRONGLY SUGGEST THAT ONLY ONE LIGAND MOLECULE IS BOUND TO A TTR TETRAMER. IN CONCLUSION, THE COORDINATES OF TWO 9CR MOLECULES ARE GIVEN, WHICH APPLYING THE CRYSTALLOGRAPHIC SYMMETRY, BECOMES FOUR, BUT ONLY ONE MUST BE CONSIDERED STATISTICALLY PRESENT IN THE TETRAMER. |
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Components
#1: Protein | Mass: 13777.360 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.39 % | |||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, sitting drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: SIEMENS-NICOLET X100 / Detector: AREA DETECTOR / Date: Nov 1, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Redundancy: 3 % / Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Resolution: 1.8→9 Å / σ(F): 0 Details: THE ENTRY CONTAINS RETINOIC ACID MOLECULES IN BOTH BINDING SITES. THE OCCUPANCIES OF SITES WERE NOT REFINED, SINCE THE ELECTRON DENSITY REFLECTS THE PRESENCE OF THE CRYSTALLOGRAPHIC TWO-FOLD ...Details: THE ENTRY CONTAINS RETINOIC ACID MOLECULES IN BOTH BINDING SITES. THE OCCUPANCIES OF SITES WERE NOT REFINED, SINCE THE ELECTRON DENSITY REFLECTS THE PRESENCE OF THE CRYSTALLOGRAPHIC TWO-FOLD AXIS. IN THE FUNCTIONAL TETRAMER THE RETINOIC ACID MOLECULES ARE DISORDERED DUE TO THE CRYSTALLOGRAPHIC TWO-FOLD RUNNING THROUGH THE BINDING SITE. RESIDUES 1 - 9 OF BOTH CHAINS, AS WELL AS 126 - 127 OF CHAIN B, ARE ILL-DEFINED IN THE ELECTRON DENSITY. THEY HAVE BEEN INCLUDED, BUT THE TEMPERATURE FACTORS ARE VERY HIGH.
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Refinement step | Cycle: LAST / Resolution: 1.8→9 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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