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- PDB-1tlk: X-RAY STRUCTURE DETERMINATION OF TELOKIN, THE C-TERMINAL DOMAIN O... -

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Basic information

Entry
Database: PDB / ID: 1tlk
TitleX-RAY STRUCTURE DETERMINATION OF TELOKIN, THE C-TERMINAL DOMAIN OF MYOSIN LIGHT CHAIN KINASE, AT 2.8 ANGSTROMS RESOLUTION
ComponentsTELOKIN
KeywordsCALMODULIN-BINDING
Function / homology
Function and homology information


tonic smooth muscle contraction / myosin light chain kinase activity / cleavage furrow / stress fiber / lamellipodium / calmodulin binding / cytoplasm
Similarity search - Function
Immunoglobulin I-set / Immunoglobulin I-set domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold ...Immunoglobulin I-set / Immunoglobulin I-set domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Biological speciesMeleagris gallopavo (turkey)
MethodX-RAY DIFFRACTION / Resolution: 2.8 Å
AuthorsHolden, H.M. / Rayment, I.
CitationJournal: J.Mol.Biol. / Year: 1992
Title: X-ray structure determination of telokin, the C-terminal domain of myosin light chain kinase, at 2.8 A resolution.
Authors: Holden, H.M. / Ito, M. / Hartshorne, D.J. / Rayment, I.
History
DepositionJul 20, 1992Processing site: BNL
Revision 1.0Oct 31, 1993Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4Feb 14, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: TELOKIN


Theoretical massNumber of molelcules
Total (without water)16,9601
Polymers16,9601
Non-polymers00
Water55831
1
A: TELOKIN

A: TELOKIN


Theoretical massNumber of molelcules
Total (without water)33,9212
Polymers33,9212
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation4_556y,x,-z+11
Unit cell
Length a, b, c (Å)64.400, 64.400, 59.600
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221
Atom site foot note1: RESIDUE PRO 69 IS A CIS PROLINE.

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Components

#1: Protein TELOKIN


Mass: 16960.326 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Meleagris gallopavo (turkey) / References: UniProt: P56276, EC: 2.7.1.117
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 31 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.1 Å3/Da / Density % sol: 41.5 %
Crystal grow
*PLUS
Temperature: 4 ℃ / Method: batch method / PH range low: 7 / PH range high: 4.5
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-ID
18 %PEG800011
20.5 mMsodium azide11
350 mMsuccinic acid11
47-12 mg(telokin/ml)potassium hydroxide11
51 mMdithiothreitol11
610 mMpotassium phosphate11

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Data collection

Reflection
*PLUS
Highest resolution: 2.7 Å / Lowest resolution: 30 Å / Num. obs: 4061 / Num. measured all: 15144 / Rmerge(I) obs: 0.05

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Processing

SoftwareName: TNT / Classification: refinement
RefinementResolution: 2.8→8 Å / Rfactor obs: 0.184
Refinement stepCycle: LAST / Resolution: 2.8→8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms806 0 0 31 837
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONt_bond_d0.011
X-RAY DIFFRACTIONt_angle_deg3
X-RAY DIFFRACTIONt_dihedral_angle_d
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes
X-RAY DIFFRACTIONt_gen_planes
X-RAY DIFFRACTIONt_it
X-RAY DIFFRACTIONt_nbd
Refinement
*PLUS
Highest resolution: 2.8 Å / Lowest resolution: 8 Å / Num. reflection obs: 3722 / Rfactor obs: 0.184
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-IDType
X-RAY DIFFRACTIONt_angle_d
X-RAY DIFFRACTIONt_angle_deg

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