+Open data
-Basic information
Entry | Database: PDB / ID: 1tkv | |||||||||
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Title | Solution Structure of T4 AsiA Dimer | |||||||||
Components | 10 kDa anti-sigma factor | |||||||||
Keywords | TRANSCRIPTION / anti-sigma / homodimer | |||||||||
Function / homology | Anti-sigma factor AsiA / Anti-Sigma Factor A / Anti-Sigma Factor A / Anti-Sigma Factor A superfamily / Anti-Sigma Factor A / regulation of DNA-templated transcription / Orthogonal Bundle / Mainly Alpha / 10 kDa anti-sigma factor Function and homology information | |||||||||
Biological species | Enterobacteria phage T4 (virus) | |||||||||
Method | SOLUTION NMR / dynamical simulated annealing, restrained energy minimization | |||||||||
Authors | Lambert, L.J. / Schirf, V. / Demeler, B. / Cadene, M. / Werner, M.H. | |||||||||
Citation | Journal: Embo J. / Year: 2001 Title: Flipping a genetic switch by subunit exchange Authors: Lambert, L.J. / Schirf, V. / Demeler, B. / Cadene, M. / Werner, M.H. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tkv.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1tkv.ent.gz | 1.1 MB | Display | PDB format |
PDBx/mmJSON format | 1tkv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1tkv_validation.pdf.gz | 368.8 KB | Display | wwPDB validaton report |
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Full document | 1tkv_full_validation.pdf.gz | 598.4 KB | Display | |
Data in XML | 1tkv_validation.xml.gz | 68.2 KB | Display | |
Data in CIF | 1tkv_validation.cif.gz | 97.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tk/1tkv ftp://data.pdbj.org/pub/pdb/validation_reports/tk/1tkv | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10604.011 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Genus: T4-like viruses / Species: Enterobacteria phage T4 sensu lato / Gene: ASIA / Plasmid: pET-28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-SI / References: UniProt: P32267 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 10 mM sodium phosphate, 50 mM NaCl, 2 mM CHAPS, 50 mM sodium azide Solvent system: 97% H2O, 3% D2O |
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Sample conditions | Ionic strength: 50 mM NaCl / pH: 6.2 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: dynamical simulated annealing, restrained energy minimization Software ordinal: 1 Details: Initial folding from a fully extended template using NOEs, dihedral restraints and NH residual dipolar couplings. Iterative refinement with NOESY data. | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry,structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 23 |