+Open data
-Basic information
Entry | Database: PDB / ID: 1tg0 | ||||||
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Title | 0.97-A structure of the SH3 domain of bbc1 | ||||||
Components | Myosin tail region-interacting protein MTI1 | ||||||
Keywords | CONTRACTILE PROTEIN / yeast / sh3 domain / structural genomics | ||||||
Function / homology | Function and homology information myosin I binding / actin cortical patch / cytoskeleton organization / actin cytoskeleton organization / nucleolus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 0.97 Å | ||||||
Authors | Kursula, P. / Kursula, I. / Lehmann, F. / Song, Y.H. / Wilmanns, M. | ||||||
Citation | Journal: To be Published Title: Saccharomyces cerevisiae SH3 domain structural genomics Authors: Kursula, P. / Kursula, I. / Lehmann, F. / Song, Y.H. / Wilmanns, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tg0.cif.gz | 51.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tg0.ent.gz | 36.9 KB | Display | PDB format |
PDBx/mmJSON format | 1tg0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tg/1tg0 ftp://data.pdbj.org/pub/pdb/validation_reports/tg/1tg0 | HTTPS FTP |
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-Related structure data
Related structure data | 1wdxS 1r6s S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7821.465 Da / Num. of mol.: 1 / Fragment: sh3 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Escherichia coli (E. coli) / References: UniProt: P47068 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 43.8 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 3.2M ammonium sulfate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7A / Wavelength: 0.9192 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jan 23, 2004 / Details: mirrors |
Radiation | Monochromator: crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9192 Å / Relative weight: 1 |
Reflection | Resolution: 0.97→28 Å / Num. obs: 39611 / Observed criterion σ(I): -3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1WDX Resolution: 0.97→20 Å / Num. parameters: 7459 / Num. restraintsaints: 10136 / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Refine analyze | Num. disordered residues: 17 / Occupancy sum hydrogen: 480.08 / Occupancy sum non hydrogen: 715.56 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 0.97→20 Å
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Refine LS restraints |
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