+Open data
-Basic information
Entry | Database: PDB / ID: 1sw6 | ||||||
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Title | S. CEREVISIAE SWI6 ANKYRIN-REPEAT FRAGMENT | ||||||
Components | REGULATORY PROTEIN SWI6 | ||||||
Keywords | TRANSCRIPTION REGULATION / ANKYRIN REPEATS / CELL-CYCLE | ||||||
Function / homology | Function and homology information SBF transcription complex / MBF transcription complex / positive regulation of reciprocal meiotic recombination / G1/S transition of mitotic cell cycle / cellular response to heat / transcription coactivator activity / regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / DNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.1 Å | ||||||
Authors | Foord, R. / Taylor, I.A. / Sedgwick, S.G. / Smerdon, S.J. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1999 Title: X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function. Authors: Foord, R. / Taylor, I.A. / Sedgwick, S.G. / Smerdon, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sw6.cif.gz | 108.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sw6.ent.gz | 88.4 KB | Display | PDB format |
PDBx/mmJSON format | 1sw6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sw6_validation.pdf.gz | 375.1 KB | Display | wwPDB validaton report |
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Full document | 1sw6_full_validation.pdf.gz | 385.1 KB | Display | |
Data in XML | 1sw6_validation.xml.gz | 12 KB | Display | |
Data in CIF | 1sw6_validation.cif.gz | 18.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sw/1sw6 ftp://data.pdbj.org/pub/pdb/validation_reports/sw/1sw6 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.790696, -0.605652, 0.089357), Vector: |
-Components
#1: Protein | Mass: 36121.246 Da / Num. of mol.: 2 / Fragment: ANKYRIN-REPEAT DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: SWI6 / Plasmid: PET22B / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21DE3 / References: UniProt: P09959 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 / Details: pH 8.50 | ||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 40 % | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / Method: vapor diffusion, hanging drop / PH range low: 8.7 / PH range high: 8.5 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, Hamburg / Beamline: X31 / Wavelength: 0.9, 0.9795, 0.9799 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 3.5→20 Å / Num. obs: 8625 / % possible obs: 99.6 % / Redundancy: 6.5 % / Rmerge(I) obs: 0.119 / Net I/σ(I): 12 | ||||||||||||
Reflection | *PLUS Highest resolution: 3.5 Å / Lowest resolution: 20 Å / Redundancy: 6.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.1→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 42.14 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→15 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.226 / Rfactor Rfree: 0.279 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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