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- PDB-1sgl: The three-dimensional structure and X-ray sequence reveal that tr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1sgl | ||||||
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Title | The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease | ||||||
![]() | trichomaglin | ||||||
![]() | HYDROLASE / trichomaglin / S-like ribonuclease / X-ray sequence / mass spectroscopic analysis | ||||||
Function / homology | ![]() ribonuclease T2 activity / Hydrolases; Acting on ester bonds / RNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gan, J.-H. / Yu, L. / Wu, J. / Xu, H. / Choudhary, J.S. / Blackstock, W.P. / Liu, W.-Y. / Xia, Z.-X. | ||||||
![]() | ![]() Title: The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease. Authors: Gan, J.H. / Yu, L. / Wu, J. / Xu, H. / Choudhary, J.S. / Blackstock, W.P. / Liu, W.Y. / Xia, Z.X. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50.4 KB | Display | ![]() |
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PDB format | ![]() | 39.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.3 KB | Display | ![]() |
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Full document | ![]() | 444.2 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 13.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23552.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() References: UniProt: P84146, UniProt: E0CX00*PLUS, Hydrolases; Acting on ester bonds | ||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.92 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: PEG 4000, tris-hydrochloric acid, potassium hydrogen sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: SEALED TUBE / Wavelength: 1.5418 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→23.56 Å / Num. all: 14893 / Num. obs: 14868 / % possible obs: 99.8 % / Observed criterion σ(F): 2 / Biso Wilson estimate: 19.8 Å2 / Rsym value: 0.1 / Net I/σ(I): 4609.6 |
Reflection shell | Resolution: 2.2→2.25 Å / Mean I/σ(I) obs: 575.1 / Num. unique all: 982 / Rsym value: 0.662 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 42.8823 Å2 / ksol: 0.338695 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→23.56 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.28 Å / Rfactor Rfree error: 0.028 / Total num. of bins used: 10
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Xplor file |
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