+Open data
-Basic information
Entry | Database: PDB / ID: 1s0v | ||||||
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Title | Structural basis for substrate selection by T7 RNA polymerase | ||||||
Components |
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Keywords | Transferase/DNA-RNA HYBRID / T7 RNA polymerase / DNA / RNA / RIKEN Structural Genomics/Proteomics Initiative / RSGI / Structural Genomics / Transferase-DNA-RNA COMPLEX / Transferase-DNA-RNA HYBRID complex | ||||||
Function / homology | Function and homology information DNA-templated viral transcription / DNA-directed RNA polymerase complex / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / DNA-templated transcription / DNA binding Similarity search - Function | ||||||
Biological species | Enterobacteria phage T7 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | ||||||
Authors | Temiakov, D. / Patlan, V. / Anikin, M. / McAllister, W.T. / Yokoyama, S. / Vassylyev, D.G. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2004 Title: Structural basis for substrate selection by t7 RNA polymerase. Authors: Temiakov, D. / Patlan, V. / Anikin, M. / McAllister, W.T. / Yokoyama, S. / Vassylyev, D.G. #1: Journal: Nature / Year: 2002 Title: Structure of a T7 RNA polymerase elongation complex at 2.9 A resolution. Authors: Tahirov, T.H. / Temiakov, D. / Anikin, M. / Patlan, V. / G Vassylyev, D. / McAllister, W.T. / Yokoyama, S. #2: Journal: Acta Crystallogr.,Sect.D / Year: 2003 Title: Crystallization and preliminary crystallographic analysis of T7 RNA polymerase elongation complex Authors: Temiakov, D. / Tahirov, T.H. / Anikin, M. / McAllister, W.T. / Vassylyev, D.G. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1s0v.cif.gz | 783.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1s0v.ent.gz | 622.7 KB | Display | PDB format |
PDBx/mmJSON format | 1s0v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s0/1s0v ftp://data.pdbj.org/pub/pdb/validation_reports/s0/1s0v | HTTPS FTP |
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-Related structure data
Related structure data | 1h83S S: Starting model for refinement |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
-DNA chain , 2 types, 8 molecules EHKNGJMP
#1: DNA chain | Mass: 5541.591 Da / Num. of mol.: 4 / Source method: obtained synthetically / Details: DNA Template strand #3: DNA chain | Mass: 2995.967 Da / Num. of mol.: 4 / Source method: obtained synthetically / Details: DNA Non-template strand |
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-RNA chain / Protein , 2 types, 8 molecules FILOABCD
#2: RNA chain | Mass: 3851.360 Da / Num. of mol.: 4 / Source method: obtained synthetically / Details: RNA transcript #4: Protein | Mass: 98984.227 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T7 (virus) / Genus: T7-like viruses / Gene: 1 / Production host: Escherichia coli (E. coli) / Strain (production host): dcat4 / References: UniProt: P00573, DNA-directed RNA polymerase |
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-Non-polymers , 3 types, 1011 molecules
#5: Chemical | ChemComp-MG / #6: Chemical | ChemComp-APC / #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.28 Å3/Da / Density % sol: 62.22 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 293 K / Method: vapor diffusion, sitting dropDetails: Temiakov, D., (2003) Acta Crystallogr.,Sect.D, 59, 185. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Type: SPRING-8 / Wavelength: 1 Å |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→40 Å / Num. all: 106154 / Num. obs: 94902 / % possible obs: 89.4 % / Observed criterion σ(F): -1 / Observed criterion σ(I): -1 |
Reflection shell | Resolution: 3→3.11 Å / % possible all: 83.9 |
Reflection | *PLUS Lowest resolution: 40 Å / Num. measured all: 229915 / Rmerge(I) obs: 0.084 |
Reflection shell | *PLUS % possible obs: 83.9 % / Rmerge(I) obs: 0.461 / Mean I/σ(I) obs: 2.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1H83 Resolution: 3.2→40 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 3.2→40 Å
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Refinement | *PLUS Lowest resolution: 40 Å / % reflection Rfree: 4 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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