[English] 日本語
![](img/lk-miru.gif)
- PDB-1s0p: Structure of the N-Terminal Domain of the Adenylyl Cyclase-Associ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1s0p | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Structure of the N-Terminal Domain of the Adenylyl Cyclase-Associated Protein (CAP) from Dictyostelium discoideum. | |||||||||
![]() | Adenylyl cyclase-associated protein | |||||||||
![]() | MEMBRANE PROTEIN / Alpha helix bundle | |||||||||
Function / homology | ![]() macropinosome / pinocytosis / contractile vacuole organization / Neutrophil degranulation / aggregation involved in sorocarp development / vacuolar membrane / response to osmotic stress / establishment or maintenance of cell polarity / pseudopodium / phagocytic cup ...macropinosome / pinocytosis / contractile vacuole organization / Neutrophil degranulation / aggregation involved in sorocarp development / vacuolar membrane / response to osmotic stress / establishment or maintenance of cell polarity / pseudopodium / phagocytic cup / adenylate cyclase binding / mitotic cytokinesis / cAMP-mediated signaling / extracellular matrix / actin filament organization / cell morphogenesis / cell-cell adhesion / phagocytic vesicle membrane / actin binding / cell cortex / regulation of gene expression / vesicle / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Ksiazek, D. / Brandstetter, H. / Israel, L. / Bourenkov, G.P. / Katchalova, G. / Janssen, K.P. / Bartunik, H.D. / Noegel, A.A. / Schleicher, M. / Holak, T.A. | |||||||||
![]() | ![]() Title: STRUCTURE OF THE N-TERMINAL DOMAIN OF THE ADENYLYL CYCLASE-ASSOCIATED PROTEIN (CAP) FROM DICTYOSTELIUM DISCOIDEUM Authors: Ksiazek, D. / Brandstetter, H. / Israel, L. / Bourenkov, G.P. / Katchalova, G. / Janssen, K.P. / Bartunik, H.D. / Noegel, A.A. / Schleicher, M. / Holak, T.A. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 95.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 71.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 374.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 389.2 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 19678.219 Da / Num. of mol.: 2 / Fragment: N-terminal domain (residues 51-226) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MG / | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.63 % | |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.1 Details: 30% PEG8000, 0.2 M MgCl2, 10 mM -mercaptoethanol, and 0.1 M MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() | ||||||||||||
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Apr 30, 2002 | ||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
| ||||||||||||
Reflection | Resolution: 1.4→1.8 Å / Num. all: 58737 / Num. obs: 20092 / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 | ||||||||||||
Reflection shell | Resolution: 1.4→1.8 Å | ||||||||||||
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 58737 / % possible obs: 93.7 % / Num. measured all: 3602228 / Rmerge(I) obs: 0.032 | ||||||||||||
Reflection shell | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 1.42 Å / % possible obs: 92.9 % / Rmerge(I) obs: 0.128 / Mean I/σ(I) obs: 4.6 |
-
Processing
Software |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.4→1.8 Å
| |||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Num. reflection obs: 3602228 / Rfactor Rwork: 0.184 | |||||||||||||||
Solvent computation | *PLUS | |||||||||||||||
Displacement parameters | *PLUS |