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Yorodumi- PDB-1rls: CRYSTAL STRUCTURE OF RNASE T1 COMPLEXED WITH THE PRODUCT NUCLEOTI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rls | ||||||
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Title | CRYSTAL STRUCTURE OF RNASE T1 COMPLEXED WITH THE PRODUCT NUCLEOTIDE 3'-GMP. STRUCTURAL EVIDENCE FOR DIRECT INTERACTION OF HISTIDINE 40 AND GLUTAMIC ACID 58 WITH THE 2'-HYDROXYL GROUP OF RIBOSE | ||||||
Components | RIBONUCLEASE T1 | ||||||
Keywords | HYDROLASE(ENDORIBONUCLEASE) | ||||||
Function / homology | Function and homology information hyphal tip / ribonuclease T1 activity / ribonuclease T1 / cell septum / endonuclease activity / lyase activity / RNA binding Similarity search - Function | ||||||
Biological species | Aspergillus oryzae (mold) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Gohda, K. / Oka, K.-I. / Tomita, K.-I. / Hakoshima, T. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 1994 Title: Crystal structure of RNase T1 complexed with the product nucleotide 3'-GMP. Structural evidence for direct interaction of histidine 40 and glutamic acid 58 with the 2'-hydroxyl group of the ribose. Authors: Gohda, K. / Oka, K. / Tomita, K. / Hakoshima, T. #1: Journal: FEBS Lett. / Year: 1985 Title: Three-Dimensional Structure of the Ribonuclease T1 X 3'-Guanylic Acid Complex at 2.6 Angstroms Resolution Authors: Sugio, S. / Oka, K. / Ohishi, H. / Tomita, K. / Saenger, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rls.cif.gz | 30.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rls.ent.gz | 23.2 KB | Display | PDB format |
PDBx/mmJSON format | 1rls.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rls_validation.pdf.gz | 456.8 KB | Display | wwPDB validaton report |
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Full document | 1rls_full_validation.pdf.gz | 458.6 KB | Display | |
Data in XML | 1rls_validation.xml.gz | 4.2 KB | Display | |
Data in CIF | 1rls_validation.cif.gz | 6.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rl/1rls ftp://data.pdbj.org/pub/pdb/validation_reports/rl/1rls | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 39 / 2: CIS PROLINE - PRO 55 |
-Components
#1: Protein | Mass: 11093.644 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aspergillus oryzae (mold) / References: UniProt: P00651, EC: 3.1.27.3 |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-3GP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 44.99 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 15 ℃ / pH: 4 / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
-Processing
Software |
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Refinement | Resolution: 1.9→8 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT/X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.167 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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