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- PDB-1rhg: THE STRUCTURE OF GRANULOCYTE-COLONY-STIMULATING FACTOR AND ITS RE... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rhg | ||||||
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Title | THE STRUCTURE OF GRANULOCYTE-COLONY-STIMULATING FACTOR AND ITS RELATIONSHIP TO THOSE OF OTHER GROWTH FACTORS | ||||||
![]() | GRANULOCYTE COLONY-STIMULATING FACTOR | ||||||
![]() | GROWTH FACTOR | ||||||
Function / homology | ![]() granulocyte colony-stimulating factor receptor binding / granulocyte colony-stimulating factor signaling pathway / positive regulation of myeloid cell differentiation / granulocyte differentiation / regulation of actin filament organization / Other interleukin signaling / positive regulation of actin filament polymerization / cellular response to cytokine stimulus / Interleukin-10 signaling / Signaling by CSF3 (G-CSF) ...granulocyte colony-stimulating factor receptor binding / granulocyte colony-stimulating factor signaling pathway / positive regulation of myeloid cell differentiation / granulocyte differentiation / regulation of actin filament organization / Other interleukin signaling / positive regulation of actin filament polymerization / cellular response to cytokine stimulus / Interleukin-10 signaling / Signaling by CSF3 (G-CSF) / endocytic vesicle lumen / lysosomal lumen / cytokine activity / growth factor activity / Inactivation of CSF3 (G-CSF) signaling / cytokine-mediated signaling pathway / positive regulation of peptidyl-tyrosine phosphorylation / cellular response to lipopolysaccharide / response to ethanol / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / immune response / positive regulation of cell population proliferation / enzyme binding / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Hill, C.P. / Osslund, T.D. / Eisenberg, D. | ||||||
![]() | ![]() Title: The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors. Authors: Hill, C.P. / Osslund, T.D. / Eisenberg, D. #1: ![]() Title: Towards Automated Crystallization Authors: Osslund, T.D. / Luthy, R. / Cascio, D. / Eisenberg, D. #2: ![]() Title: Folding and Oxidation of Recombinant Human Granulocyte Colony Stimulating Factor Produced in Escherichia Coli. Characterization of the Disulfide-Reduced Intermediates and Cysteine-Serine Analogs Authors: Lu, H.S. / Clogston, C.L. / Narhi, L.O. / Merewether, L.A. / Pearl, W.R. / Boone, T.C. #3: ![]() Title: Recombinant Human Granulocyte Colony-Stimulating Factor: Effects on Normal and Leukemic Myeloid Cells Authors: Souza, L.M. / Boone, T.C. / Gabrilove, J. / Lai, P.H. / Zsebo, K.M. / Murdock, D.C. / Chazin, V.R. / Bruszewski, J. / Lu, H. / Chen, K.C. / Barendt, J. / Platzer, E. / Moore, M.A.S. / Mertelsmann, R. / Welte, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.4 KB | Display | ![]() |
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PDB format | ![]() | 85.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 408 KB | Display | ![]() |
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Full document | ![]() | 420.1 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 16.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 18685.564 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 44.59 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 5.8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.2 Å / Num. obs: 10735 / % possible obs: 93.3 % / Num. measured all: 50868 / Rmerge(I) obs: 0.056 |
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Processing
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Refinement | Rfactor Rwork: 0.215 / Rfactor obs: 0.215 / Highest resolution: 2.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.2 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 6 Å / Rfactor obs: 0.215 / Rfactor Rfree: 0.344 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 44 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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