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Yorodumi- PDB-1rgl: RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rgl | ||||||
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Title | RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT THE 3' SUBSITE | ||||||
Components | RIBONUCLEASE T1 | ||||||
Keywords | HYDROLASE(ENDORIBONUCLEASE) | ||||||
Function / homology | Function and homology information hyphal tip / ribonuclease T1 activity / ribonuclease T1 / cell septum / endonuclease activity / lyase activity / RNA binding Similarity search - Function | ||||||
Biological species | Aspergillus oryzae (mold) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Granzin, J. / Puras-Lutzke, R. / Landt, O. / Grunert, H.-P. / Heinemann, U. / Saenger, W. / Hahn, U. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1992 Title: RNase T1 mutant Glu46Gln binds the inhibitors 2'GMP and 2'AMP at the 3' subsite. Authors: Granzin, J. / Puras-Lutzke, R. / Landt, O. / Grunert, H.P. / Heinemann, U. / Saenger, W. / Hahn, U. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rgl.cif.gz | 30 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rgl.ent.gz | 22.5 KB | Display | PDB format |
PDBx/mmJSON format | 1rgl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rgl_validation.pdf.gz | 797.5 KB | Display | wwPDB validaton report |
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Full document | 1rgl_full_validation.pdf.gz | 797.5 KB | Display | |
Data in XML | 1rgl_validation.xml.gz | 7.4 KB | Display | |
Data in CIF | 1rgl_validation.cif.gz | 9.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rg/1rgl ftp://data.pdbj.org/pub/pdb/validation_reports/rg/1rgl | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO 39 AND PRO 55 ARE CIS PROLINES. |
-Components
#1: Protein | Mass: 11093.710 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aspergillus oryzae (mold) / Production host: Escherichia coli (E. coli) / References: UniProt: P00651, EC: 3.1.27.3 |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-2GP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.19 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Method: microdialysis | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 10 Å / Num. obs: 5721 / % possible obs: 85 % / Observed criterion σ(F): 1 / Num. measured all: 11009 / Rmerge(I) obs: 0.03 |
-Processing
Software | Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.148 / Highest resolution: 2 Å | ||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS
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