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- PDB-1rf8: Solution structure of the yeast translation initiation factor eIF... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rf8 | |||||||||
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Title | Solution structure of the yeast translation initiation factor eIF4E in complex with m7GDP and eIF4GI residues 393 to 490 | |||||||||
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![]() | BIOSYNTHETIC PROTEIN / TRANSLATION / Initiation factor / Protein biosynthesis / Translation regulation | |||||||||
Function / homology | ![]() Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / positive regulation of formation of translation preinitiation complex / Deadenylation of mRNA / eukaryotic translation initiation factor 4F complex / mRNA cap binding / regulation of protein metabolic process / RNA 7-methylguanosine cap binding / phosphatidylinositol-3-phosphate binding / positive regulation of endoplasmic reticulum unfolded protein response / mTORC1-mediated signalling ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / positive regulation of formation of translation preinitiation complex / Deadenylation of mRNA / eukaryotic translation initiation factor 4F complex / mRNA cap binding / regulation of protein metabolic process / RNA 7-methylguanosine cap binding / phosphatidylinositol-3-phosphate binding / positive regulation of endoplasmic reticulum unfolded protein response / mTORC1-mediated signalling / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / Translation initiation complex formation / Ribosomal scanning and start codon recognition / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ATPase activator activity / L13a-mediated translational silencing of Ceruloplasmin expression / stress granule assembly / translational initiation / translation initiation factor activity / molecular condensate scaffold activity / ribosomal large subunit biogenesis / P-body / cytoplasmic stress granule / : / ribosome / regulation of cell cycle / mRNA binding / mitochondrion / RNA binding / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | SOLUTION NMR | |||||||||
![]() | Gross, J.D. / Moerke, N.J. / von der Haar, T. / Lugovskoy, A.A. / Sachs, A.B. / McCarthy, J.E.G. / Wagner, G. | |||||||||
![]() | ![]() Title: Ribosome loading onto the mRNA cap is driven by conformational coupling between eIF4G and eIF4E. Authors: Gross, J.D. / Moerke, N.J. / von der Haar, T. / Lugovskoy, A.A. / Sachs, A.B. / McCarthy, J.E. / Wagner, G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 910.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 632.8 KB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 152.4 KB | Display | |
Data in CIF | ![]() | 197.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 24211.230 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: TIF45, CDC33, YOL139C / Plasmid: PET30a / Cell line (production host): BL21(DE3) / Production host: ![]() ![]() | ||
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#2: Protein | Mass: 11087.355 Da / Num. of mol.: 1 / Fragment: residues 391-488 (SWS:P39935) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: TIF4631, YGR162W / Plasmid: pGEX-2T / Cell line (production host): BL21 / Production host: ![]() ![]() | ||
#3: Chemical | ChemComp-MTN / #4: Chemical | ChemComp-M7G / | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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Sample preparation
Details | Contents: 0.6 mM 1:1:1 m7GDP/eIF4E/eIF4G(393-490) complex Solvent system: 50 mM Sodium Phosphate 50 mM KCl 12 mM CHAPS |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
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NMR spectrometer |
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Processing
NMR software |
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NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 11 |