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Open data
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Basic information
Entry | Database: PDB / ID: 1rcy | ||||||
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Title | RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS | ||||||
![]() | RUSTICYANIN | ||||||
![]() | METALLOPROTEIN / COPPER CONTAINING PROTEIN / OXIDATION POTENTIAL / PH STABILITY / REDOX PROTEIN | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Walter, R.L. / Friedman, A.M. / Ealick, S.E. / Blake II, R.C. / Proctor, P. / Shoham, M. | ||||||
![]() | ![]() Title: Multiple wavelength anomalous diffraction (MAD) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability. Authors: Walter, R.L. / Ealick, S.E. / Friedman, A.M. / Blake 2nd., R.C. / Proctor, P. / Shoham, M. #1: ![]() Title: Madprb: A New Suite of Programs for MAD Data Analysis Incorporating Robust Estimation, Maximum Likelihood and Bayesian Inference Authors: Friedman, A.M. / Fischmann, T.O. / Shamoo, Y. / Ealick, S.E. #2: ![]() Title: Complete 13C Assignments for Recombinant Cu(I) Rusticyanin. Prediction of Secondary Structure from Patterns of Chemical Shifts Authors: Toy-Palmer, A. / Prytulla, S. / Dyson, H.J. #3: ![]() Title: X-Ray Absorption Studies and Homology Modeling Define the Structural Features that Specify the Nature of the Copper Site in Rusticyanin Authors: Grossmann, J.G. / Ingledew, W.J. / Harvey, I. / Strange, R.W. / Hasnain, S.S. #4: ![]() Title: Nuclear Magnetic Resonance 15N and 1H Resonance Assignments and Global Fold of Rusticyanin. Insights Into the Ligation and Acid Stability of the Blue Copper Site Authors: Hunt, A.H. / Toy-Palmer, A. / Assa-Munt, N. / Cavanagh, J. / Blake II, R.C. / Dyson, H.J. #5: ![]() Title: Crystallization and Preliminary X-Ray Crystallographic Studies of Rusticyanin from Thiobacillus Ferrooxidans Authors: Djebli, A. / Proctor, P. / Blake II, R.C. / Shoham, M. #6: ![]() Title: Respiratory Components in Acidophilic Bacteria that Respire on Iron Authors: Blake II, R.C. / Shute, E.A. / Waskovsky, J. / Harrison Junior, A.P. #7: ![]() Title: Amino Acid Sequence of the Blue Copper Protein Rusticyanin from Thiobacillus Ferrooxidans Authors: Ronk, M. / Shively, J.E. / Shute, E.A. / Blake II, R.C. #9: ![]() Title: Respiratory Enzymes of Thiobacillus Ferrooxidans. A Kinetic Study of Electron Transfer between Iron and Rusticyanin in Sulfate Media Authors: Blake II, R.C. / Shute, E.A. #10: ![]() Title: The Purification and Some Properties of Rusticyanin, a Blue Copper Protein Involved in Iron(II) Oxidation from Thiobacillus Ferro-Oxidans Authors: Cox, J.C. / Boxer, D.H. #11: ![]() Title: The Respiratory Chain of Thiobacillus Ferrooxidans: The Reduction of Cytochromes by Fe2+ and the Preliminary Characterization of Rusticyanin, a Novel 'Blue' Copper Protein Authors: Cobley, J.G. / Haddock, B.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45 KB | Display | ![]() |
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PDB format | ![]() | 30.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.7 KB | Display | ![]() |
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Full document | ![]() | 421.1 KB | Display | |
Data in XML | ![]() | 9.2 KB | Display | |
Data in CIF | ![]() | 12.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 16197.503 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: OXIDIZED FORM (CU(II)) Source: (gene. exp.) ![]() Cellular location: PERIPLASM / References: UniProt: P24930, UniProt: P0C918*PLUS |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.6 / Details: pH 4.6 | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop / Details: Djebli, A., (1992) J.Mol.Biol., 227, 581. | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Oct 20, 1993 / Details: COLLIMATOR |
Radiation | Monochromator: SI(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.9 Å / Num. obs: 9167 / % possible obs: 82 % / Observed criterion σ(I): 2 / Redundancy: 1.5 % / Rmerge(I) obs: 0.02 / Net I/σ(I): 23.5 |
Reflection shell | Resolution: 1.9→2.05 Å / Redundancy: 1.1 % / Rmerge(I) obs: 0.0383 / Mean I/σ(I) obs: 10.1 / % possible all: 63 |
Reflection | *PLUS Num. measured all: 14468 |
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Processing
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Refinement | Method to determine structure: MULTIWAVELENGTH ANOMALOUS DISPERSION Resolution: 1.9→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 14.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.99 Å / % reflection obs: 50 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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