+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1rcb | ||||||
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タイトル | CRYSTAL STRUCTURE OF HUMAN RECOMBINANT INTERLEUKIN-4 AT 2.25 ANGSTROMS RESOLUTION | ||||||
要素 | INTERLEUKIN-4 | ||||||
キーワード | CYTOKINE | ||||||
機能・相同性 | 機能・相同性情報 interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / negative regulation of complement-dependent cytotoxicity / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling / negative regulation of neuroinflammatory response / negative regulation of epithelial cell migration / positive regulation of T-helper 2 cell cytokine production / dendritic cell differentiation ...interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / negative regulation of complement-dependent cytotoxicity / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling / negative regulation of neuroinflammatory response / negative regulation of epithelial cell migration / positive regulation of T-helper 2 cell cytokine production / dendritic cell differentiation / positive regulation of isotype switching to IgG isotypes / neuroinflammatory response / interleukin-4-mediated signaling pathway / macrophage activation / positive regulation of interleukin-13 production / myeloid dendritic cell differentiation / positive regulation of amyloid-beta clearance / regulation of phosphorylation / type 2 immune response / activation of Janus kinase activity / positive regulation of MHC class II biosynthetic process / negative regulation of cellular response to transforming growth factor beta stimulus / positive regulation of T cell differentiation / positive regulation of ATP biosynthetic process / negative regulation of osteoclast differentiation / positive regulation of interleukin-10 production / positive regulation of macroautophagy / negative regulation of tumor necrosis factor production / regulation of immune response / negative regulation of endothelial cell apoptotic process / positive regulation of T cell proliferation / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / T cell activation / cholesterol metabolic process / B cell differentiation / cytokine activity / growth factor activity / negative regulation of inflammatory response / positive regulation of receptor-mediated endocytosis / positive regulation of cold-induced thermogenesis / Interleukin-4 and Interleukin-13 signaling / positive regulation of cell migration / immune response / negative regulation of DNA-templated transcription / positive regulation of cell population proliferation / positive regulation of gene expression / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / 解像度: 2.25 Å | ||||||
データ登録者 | Wlodawer, A. / Pavlovsky, A. / Gustchina, A. | ||||||
引用 | ジャーナル: FEBS Lett. / 年: 1992 タイトル: Crystal structure of human recombinant interleukin-4 at 2.25 A resolution. 著者: Wlodawer, A. / Pavlovsky, A. / Gustchina, A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1rcb.cif.gz | 38.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1rcb.ent.gz | 26.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1rcb.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/rc/1rcb ftp://data.pdbj.org/pub/pdb/validation_reports/rc/1rcb | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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Atom site foot note | 1: THE ELECTRON DENSITY FOR RESIDUES 38 - 40 IS POOR, AND THE TRACING IN THAT REGION IS UNCERTAIN. |
-要素
#1: タンパク質 | 分子量: 14989.248 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 参照: UniProt: P05112 |
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#2: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 3.26 Å3/Da / 溶媒含有率: 62.28 % | |||||||||||||||||||||||||
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結晶化 | *PLUS pH: 6 / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS 最高解像度: 2.23 Å / Num. obs: 8951 / % possible obs: 90.7 % / Observed criterion σ(F): 2 / Rmerge(I) obs: 0.06 |
-解析
ソフトウェア | 名称: PROLSQ / 分類: 精密化 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 解像度: 2.25→10 Å 詳細: THE ELECTRON DENSITY FOR RESIDUES 38 - 40 IS POOR, AND THE TRACING IN THAT REGION IS UNCERTAIN.
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精密化ステップ | サイクル: LAST / 解像度: 2.25→10 Å
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拘束条件 |
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精密化 | *PLUS 最高解像度: 2.25 Å / 最低解像度: 10 Å / Num. reflection obs: 8085 / Rfactor obs: 0.218 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |