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- PDB-1r5s: Connexin 43 Carboxyl Terminal Domain -

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Basic information

Entry
Database: PDB / ID: 1r5s
TitleConnexin 43 Carboxyl Terminal Domain
ComponentsGap junction alpha-1 protein
KeywordsMEMBRANE PROTEIN / Cx43CT
Function / homology
Function and homology information


vascular transport / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Regulation of gap junction activity / positive regulation of cell communication by chemical coupling / Gap junction assembly / positive regulation of glomerular filtration / RHOQ GTPase cycle / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex ...vascular transport / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Regulation of gap junction activity / positive regulation of cell communication by chemical coupling / Gap junction assembly / positive regulation of glomerular filtration / RHOQ GTPase cycle / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / positive regulation of behavioral fear response / neuroblast migration / chronic inflammatory response / Gap junction degradation / Formation of annular gap junctions / columnar/cuboidal epithelial cell maturation / maintenance of protein localization in endoplasmic reticulum / regulation of cell communication by electrical coupling / gap junction hemi-channel activity / positive regulation of establishment of Sertoli cell barrier / negative regulation of trophoblast cell migration / positive regulation of meiotic nuclear division / regulation of bone remodeling / microtubule-based transport / monoatomic ion transmembrane transporter activity / ATP transport / regulation of ventricular cardiac muscle cell membrane depolarization / epididymis development / gap junction channel activity involved in cell communication by electrical coupling / contractile muscle fiber / glutathione transmembrane transporter activity / gap junction assembly / negative regulation of cardiac muscle cell proliferation / cellular response to pH / endothelium development / skeletal muscle tissue regeneration / response to fluid shear stress / connexin complex / regulation of atrial cardiac muscle cell membrane depolarization / cardiac conduction / regulation of actin filament organization / Golgi-associated vesicle membrane / fascia adherens / cell-cell contact zone / gap junction / gap junction channel activity / bone remodeling / export across plasma membrane / cell-cell junction organization / negative regulation of DNA biosynthetic process / adult heart development / connexin binding / xenobiotic transport / regulation of bone mineralization / embryonic heart tube development / glutamate secretion / regulation of ventricular cardiac muscle cell membrane repolarization / tight junction / response to pH / regulation of bicellular tight junction assembly / cellular response to parathyroid hormone stimulus / blood vessel morphogenesis / lens development in camera-type eye / intermediate filament / regulation of heart contraction / embryonic digit morphogenesis / beta-tubulin binding / positive regulation of stem cell proliferation / heart looping / negative regulation of endothelial cell proliferation / efflux transmembrane transporter activity / establishment of mitotic spindle orientation / regulation of calcium ion transport / decidualization / intercalated disc / lateral plasma membrane / response to glucose / T cell proliferation / response to retinoic acid / monoatomic ion transmembrane transport / positive regulation of vasoconstriction / positive regulation of vascular associated smooth muscle cell proliferation / T cell activation / multivesicular body / tubulin binding / protein tyrosine kinase binding / neuron projection morphogenesis / response to ischemia / PDZ domain binding / regulation of transmembrane transporter activity / neuron migration / bone development / protein localization / positive regulation of insulin secretion / negative regulation of cell growth
Similarity search - Function
Connexin43 / Gap junction alpha-1 protein (Cx43) / Gap junction alpha-1 protein (Cx43), C-terminal / Gap junction alpha-1 protein (Cx43), alpha helix domain superfamily / Gap junction alpha-1 protein (Cx43) / Connexin, C-terminal / Connexin / Connexin, N-terminal / Connexin, conserved site / Gap junction protein, cysteine-rich domain ...Connexin43 / Gap junction alpha-1 protein (Cx43) / Gap junction alpha-1 protein (Cx43), C-terminal / Gap junction alpha-1 protein (Cx43), alpha helix domain superfamily / Gap junction alpha-1 protein (Cx43) / Connexin, C-terminal / Connexin / Connexin, N-terminal / Connexin, conserved site / Gap junction protein, cysteine-rich domain / Connexin, N-terminal domain superfamily / Connexin / Connexins signature 1. / Connexins signature 2. / Connexin homologues / Gap junction channel protein cysteine-rich domain / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Gap junction alpha-1 protein
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodSOLUTION NMR / torsion angle dynamics, simulated annealing
AuthorsSorgen, P.L. / Duffy, H.S. / Mario, D. / Sahoo, P. / Coombs, W. / Delmar, M. / Spray, D.C.
CitationJournal: J.Biol.Chem. / Year: 2004
Title: Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1
Authors: Sorgen, P.L. / Duffy, H.S. / Sahoo, P. / Coombs, W. / Delmar, M. / Spray, D.C.
History
DepositionOct 13, 2003Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 26, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 29, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly ...database_2 / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Gap junction alpha-1 protein


Theoretical massNumber of molelcules
Total (without water)14,2171
Polymers14,2171
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / 100structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein Gap junction alpha-1 protein / Connexin 43 / Cx43 / Gap junction 43 kDa heart protein


Mass: 14217.435 Da / Num. of mol.: 1 / Fragment: Carboxyl Terminal Domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: GJA1 OR CXN-43 / Production host: Escherichia coli (E. coli) / References: UniProt: P08050

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D 13C-separated NOESY
1213D 15N-separated NOESY
NMR detailsText: This structure was determined using standard 3D homonuclear techniques

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Sample preparation

DetailsContents: 1.5mM Cx43CT U-15N,13C, PBS / Solvent system: 95% H2O/5% D2O
Sample conditionsIonic strength: PBS / pH: 5.8 / Pressure: ambient / Temperature: 280 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.1Brunger, et al.structure solution
NMRPipe7.2Delaglio, et al.processing
CNS1.1Brunger, et al.refinement
RefinementMethod: torsion angle dynamics, simulated annealing / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 10

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