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Yorodumi- PDB-1r2u: NMR structure of the N domain of trout cardiac troponin C at 30 C -
+Open data
-Basic information
Entry | Database: PDB / ID: 1r2u | ||||||
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Title | NMR structure of the N domain of trout cardiac troponin C at 30 C | ||||||
Components | troponin C | ||||||
Keywords | METAL BINDING PROTEIN / EF-hand | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Oncorhynchus mykiss (rainbow trout) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Blumenschein, T.M. / Gillis, T.E. / Tibbits, G.F. / Sykes, B.D. | ||||||
Citation | Journal: Biochemistry / Year: 2004 Title: Effect of temperature on the structure of trout troponin C Authors: Blumenschein, T.M. / Gillis, T.E. / Tibbits, G.F. / Sykes, B.D. #1: Journal: Biochemistry / Year: 2003 Title: Effect of temperature and the F27W mutation on the Ca2+ activated structural transition of trout cardiac troponin C Authors: Gillis, T.E. / Blumenschein, T.M.A. / Sykes, B.D. / Tibbits, G.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1r2u.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1r2u.ent.gz | 886.7 KB | Display | PDB format |
PDBx/mmJSON format | 1r2u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r2/1r2u ftp://data.pdbj.org/pub/pdb/validation_reports/r2/1r2u | HTTPS FTP |
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-Related structure data
Related structure data | 1r6pC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10142.326 Da / Num. of mol.: 1 / Fragment: N-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Oncorhynchus mykiss (rainbow trout) / Organ: heart / Plasmid: pGex / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q7ZZB9 |
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#2: Chemical | ChemComp-CA / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 100 mM KCl / pH: 6.9 / Pressure: 1 atm / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 60 / Conformers submitted total number: 40 |