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- PDB-1r0x: Cystic fibrosis transmembrane conductance regulator (CFTR) nucleo... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1r0x | ||||||
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Title | Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP | ||||||
![]() | Cystic fibrosis transmembrane conductance regulator | ||||||
![]() | TRANSPORT PROTEIN / ABC transporter nucleotide binding domain | ||||||
Function / homology | ![]() RHO GTPases regulate CFTR trafficking / RHOQ GTPase cycle / positive regulation of mast cell activation / transepithelial chloride transport / positive regulation of establishment of Sertoli cell barrier / Aggrephagy / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / water transport / channel-conductance-controlling ATPase ...RHO GTPases regulate CFTR trafficking / RHOQ GTPase cycle / positive regulation of mast cell activation / transepithelial chloride transport / positive regulation of establishment of Sertoli cell barrier / Aggrephagy / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / water transport / channel-conductance-controlling ATPase / intracellularly ATP-gated chloride channel activity / positive regulation of enamel mineralization / transepithelial water transport / negative regulation of vascular associated smooth muscle cell apoptotic process / enamel mineralization / intracellular pH elevation / negative regulation of type B pancreatic cell development / amelogenesis / chloride channel inhibitor activity / Cargo recognition for clathrin-mediated endocytosis / ABC-family proteins mediated transport / Clathrin-mediated endocytosis / multicellular organismal-level water homeostasis / Ub-specific processing proteases / membrane hyperpolarization / cholesterol transport / bicarbonate transport / bicarbonate transmembrane transporter activity / vesicle docking involved in exocytosis / chloride transport / chloride transmembrane transporter activity / sperm capacitation / chloride channel activity / cholesterol biosynthetic process / positive regulation of exocytosis / positive regulation of insulin secretion involved in cellular response to glucose stimulus / chloride channel complex / microvillus / sodium ion transmembrane transport / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / cellular response to cAMP / cellular response to forskolin / chloride transmembrane transport / isomerase activity / response to endoplasmic reticulum stress / establishment of localization in cell / PDZ domain binding / lung development / vasodilation / recycling endosome membrane / early endosome membrane / protein-folding chaperone binding / basolateral plasma membrane / early endosome / response to xenobiotic stimulus / apical plasma membrane / neuronal cell body / dendrite / endoplasmic reticulum membrane / enzyme binding / cell surface / ATP hydrolysis activity / ATP binding / membrane / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lewis, H.A. / Buchanan, S.G. / Burley, S.K. / Conners, K. / Dickey, M. / Dorwart, M. / Fowler, R. / Gao, X. / Guggino, W.B. / Hendrickson, W.A. | ||||||
![]() | ![]() Title: Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator. Authors: Lewis, H.A. / Buchanan, S.G. / Burley, S.K. / Conners, K. / Dickey, M. / Dorwart, M. / Fowler, R. / Gao, X. / Guggino, W.B. / Hendrickson, W.A. / Hunt, J.F. / Kearins, M.C. / Lorimer, D. / ...Authors: Lewis, H.A. / Buchanan, S.G. / Burley, S.K. / Conners, K. / Dickey, M. / Dorwart, M. / Fowler, R. / Gao, X. / Guggino, W.B. / Hendrickson, W.A. / Hunt, J.F. / Kearins, M.C. / Lorimer, D. / Maloney, P.C. / Post, K.W. / Rajashankar, K.R. / Rutter, M.E. / Sauder, J.M. / Shriver, S. / Thibodeau, P.H. / Thomas, P.J. / Zhang, M. / Zhao, X. / Emtage, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 229.3 KB | Display | ![]() |
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PDB format | ![]() | 183.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.9 MB | Display | ![]() |
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Full document | ![]() | 1.9 MB | Display | |
Data in XML | ![]() | 44.7 KB | Display | |
Data in CIF | ![]() | 61.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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5 | ![]()
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6 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32098.324 Da / Num. of mol.: 4 / Fragment: NBD1 domain (residues 389-673) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-ATP / #4: Chemical | ChemComp-ACY / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.23 Å3/Da / Density % sol: 61.91 % | |||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |||||||||||||||
Crystal grow | *PLUS Temperature: 4, 20 ℃ / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: May 16, 2003 |
Radiation | Monochromator: Silicon 220 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→39.2 Å / Num. obs: 75175 / % possible obs: 98.5 % / Rsym value: 0.073 |
Reflection shell | Resolution: 2.2→2.28 Å / Rsym value: 0.5 / % possible all: 92.7 |
Reflection | *PLUS Redundancy: 7 % / Rmerge(I) obs: 0.073 |
Reflection shell | *PLUS % possible obs: 92.7 % / Redundancy: 5.7 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.2→39.2 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor Rwork: 0.234 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |