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Yorodumi- PDB-1qwq: Solution structure of the monomeric N67D mutant of Bovine Seminal... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qwq | |||||||||
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Title | Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease | |||||||||
Components | Ribonuclease | |||||||||
Keywords | HYDROLASE / ALPHA-BETA-PROTEIN | |||||||||
Function / homology | Function and homology information pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region / identical protein binding Similarity search - Function | |||||||||
Biological species | Bos taurus (cattle) | |||||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS, ENERGY MINIMIZATION | |||||||||
Authors | Avitabile, F. / Alfano, C. / Spadaccini, R. / Crescenzi, O. / D'Ursi, A.M. / D'Alessio, G. / Tancredi, T. / Picone, D. | |||||||||
Citation | Journal: Biochemistry / Year: 2003 Title: THE SWAPPING OF TERMINAL ARMS IN RIBONUCLEASES: COMPARISON OF THE SOLUTION STRUCTURE OF MONOMERIC BOVINE SEMINAL AND PANCREATIC RIBONUCLEASES Authors: Avitabile, F. / Alfano, C. / Spadaccini, R. / Crescenzi, O. / D'Ursi, A.M. / D'Alessio, G. / Tancredi, T. / Picone, D. #1: Journal: J.BIOMOL.NMR / Year: 2001 Title: 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease. Authors: Crescenzi, O. / Carotenuto, A. / D'Ursi, A.M. / Tancredi, T. / D'Alessio, G. / Avitabile, F. / Picone, D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qwq.cif.gz | 582.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qwq.ent.gz | 504.9 KB | Display | PDB format |
PDBx/mmJSON format | 1qwq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1qwq_validation.pdf.gz | 401.7 KB | Display | wwPDB validaton report |
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Full document | 1qwq_full_validation.pdf.gz | 484 KB | Display | |
Data in XML | 1qwq_validation.xml.gz | 29.2 KB | Display | |
Data in CIF | 1qwq_validation.cif.gz | 51.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qw/1qwq ftp://data.pdbj.org/pub/pdb/validation_reports/qw/1qwq | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13633.624 Da / Num. of mol.: 1 / Mutation: N67D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Gene: SRN / Plasmid: PET-22B+ / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P00669, EC: 3.1.27.5 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 5.65 / Pressure: ambient / Temperature: 300 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: TORSION ANGLE DYNAMICS, ENERGY MINIMIZATION / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 40 / Conformers submitted total number: 16 |