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Open data
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Basic information
Entry | Database: PDB / ID: 1qwi | ||||||
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Title | Crystal Structure of E. coli OsmC | ||||||
![]() | osmotically inducible protein | ||||||
![]() | hydroperoxide reductase / hydroperoxide resistance | ||||||
Function / homology | ![]() peroxiredoxin activity / hyperosmotic response / response to hydroperoxide / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / peroxidase activity / response to oxidative stress / protein homodimerization activity / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lesniak, J. / Barton, W.A. / Nikolov, D.B. | ||||||
![]() | ![]() Title: Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC Authors: Lesniak, J. / Barton, W.A. / Nikolov, D.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 118 KB | Display | ![]() |
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PDB format | ![]() | 97.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 381 KB | Display | ![]() |
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Full document | ![]() | 386.5 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 21 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15275.818 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.26 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 5000 MME, 0.2M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Dec 1, 2001 | ||||||||||||
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.8→30 Å / Num. all: 51960 / Num. obs: 48947 / % possible obs: 94.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.053 / Rsym value: 0.045 | ||||||||||||
Reflection shell | Resolution: 1.8→1.88 Å / % possible all: 94.8 | ||||||||||||
Reflection | *PLUS % possible obs: 99.7 % / Redundancy: 2.1 % / Rmerge(I) obs: 0.039 | ||||||||||||
Reflection shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.09 Å / % possible obs: 98.7 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
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Refinement | *PLUS Lowest resolution: 500 Å / Num. reflection obs: 93520 / Rfactor Rfree: 0.24 / Rfactor Rwork: 0.22 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.09 Å |