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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1qk9 | ||||||
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タイトル | The solution structure of the domain from MeCP2 that binds to methylated DNA | ||||||
![]() | METHYL-CPG-BINDING PROTEIN 2 | ||||||
![]() | METHYL-CPG-BINDING PROTEIN / SOLUTION STRUCTURE / METHYLATED DNA / METHYL CYTOSINE / MBD | ||||||
機能・相同性 | ![]() trans-synaptic signaling by BDNF / regulation of action potential firing threshold / negative regulation of respiratory gaseous exchange / Loss of MECP2 binding ability to 5hmC-DNA / cellular response to isoquinoline alkaloid / positive regulation of anterograde dense core granule transport / positive regulation of retrograde dense core granule transport / positive regulation of branching morphogenesis of a nerve / catecholamine secretion / MECP2 regulates transcription of genes involved in GABA signaling ...trans-synaptic signaling by BDNF / regulation of action potential firing threshold / negative regulation of respiratory gaseous exchange / Loss of MECP2 binding ability to 5hmC-DNA / cellular response to isoquinoline alkaloid / positive regulation of anterograde dense core granule transport / positive regulation of retrograde dense core granule transport / positive regulation of branching morphogenesis of a nerve / catecholamine secretion / MECP2 regulates transcription of genes involved in GABA signaling / biogenic amine metabolic process / negative regulation of dendrite extension / principal sensory nucleus of trigeminal nerve development / cardiolipin metabolic process / negative regulation of locomotion involved in locomotory behavior / Loss of MECP2 binding ability to 5mC-DNA / nervous system process involved in regulation of systemic arterial blood pressure / proprioception / negative regulation of primary miRNA processing / MECP2 regulates transcription of neuronal ligands / negative regulation of smooth muscle cell differentiation / Loss of MECP2 binding ability to the NCoR/SMRT complex / negative regulation of dendritic spine development / Transcriptional Regulation by MECP2 / regulation of respiratory gaseous exchange by nervous system process / double-stranded methylated DNA binding / ventricular cardiac muscle tissue development / inositol metabolic process / genomic imprinting / phosphatidylcholine metabolic process / thalamus development / positive regulation of microtubule nucleation / glucocorticoid metabolic process / ventricular system development / cellular response to potassium ion / unmethylated CpG binding / positive regulation of synaptic plasticity / respiratory gaseous exchange by respiratory system / oligodendrocyte development / olfactory bulb development / positive regulation of dendrite extension / striatum development / response to other organism / siRNA binding / neuron maturation / methyl-CpG binding / MECP2 regulates transcription factors / Loss of phosphorylation of MECP2 at T308 / negative regulation of gene expression via chromosomal CpG island methylation / response to ionizing radiation / spinal cord development / positive regulation of dendritic spine development / regulation of synapse organization / lung alveolus development / glutamine metabolic process / startle response / dendrite development / social behavior / negative regulation of blood vessel endothelial cell migration / negative regulation of astrocyte differentiation / Regulation of MECP2 expression and activity / behavioral fear response / glial cell proliferation / heterochromatin / long-term memory / Nuclear events stimulated by ALK signaling in cancer / MECP2 regulates neuronal receptors and channels / four-way junction DNA binding / positive regulation of glial cell proliferation / Notch signaling pathway / heterochromatin formation / sensory perception of pain / synapse assembly / histone reader activity / excitatory postsynaptic potential / negative regulation of angiogenesis / adult locomotory behavior / cerebellum development / post-embryonic development / molecular condensate scaffold activity / response to cocaine / hippocampus development / promoter-specific chromatin binding / long-term synaptic potentiation / response to lead ion / visual learning / protein localization / chromatin DNA binding / cerebral cortex development / histone deacetylase binding / transcription corepressor activity / response to estradiol / gene expression / postsynapse / negative regulation of neuron apoptotic process / nucleic acid binding / molecular adaptor activity / response to hypoxia / protein domain specific binding / negative regulation of gene expression 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
![]() | Wakefield, R.I.D. / Smith, B.O. / Nan, X. / Free, A. / Soteriou, A. / Uhrin, D. / Bird, A.P. / Barlow, P.N. | ||||||
![]() | ![]() タイトル: The Solution Structure of the Domain from Mecp2 that Binds to Methylated DNA 著者: Wakefield, R.I.D. / Smith, B.O. / Nan, X. / Free, A. / Soteriou, A. / Uhrin, D. / Bird, A.P. / Barlow, P.N. #1: ジャーナル: Mol.Cell.Biol. / 年: 1998 タイトル: Identification and Characterisation of a Family of Mammalian Methyl-Cpg Binding Proteins 著者: Hendrich, B. / Bird, A.P. #2: ジャーナル: Nature / 年: 1998 タイトル: Transcriptional Repression by the Methyl-Cpg Binding Protein Mecp2 Involves a Histone Deacetylase Complex 著者: Nan, X.S. / Ng, H.-H. / Johnson, C.A. / Laherty, C.D. / Turner, B.M. / Eisenmann, R.N. / Bird, A. #4: ジャーナル: J.Mol.Biol. / 年: 1995 タイトル: Calculation of Protein Structures with Ambiguous Distance Restraints Automated Assignment of Ambiguous Noe Crosspeaks and Disulfide Connectivities 著者: Nilges, M. #5: ジャーナル: Cell(Cambridge,Mass.) / 年: 1991 タイトル: DNA Methylation Inhibits Transcription Indirectly Via a Methyl-Cpg Binding Protein 著者: Boyes, J. / Bird, A.P. | ||||||
履歴 |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 781 KB | 表示 | ![]() |
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PDB形式 | ![]() | 653.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 347.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 583.8 KB | 表示 | |
XML形式データ | ![]() | 55.9 KB | 表示 | |
CIF形式データ | ![]() | 87.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 10261.530 Da / 分子数: 1 / 断片: MCPG BINDING DOMAIN OF MECP2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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試料調製
詳細 | 内容: 90% H2O / 10% D2O |
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試料状態 | pH: 6.0 / 温度: 291 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 600 MHz |
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解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: AMBIGUOUS DISTANCE RESTRAINTS WERE USED SEE M.NILGES CITATION | ||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LOWEST ENERGIES / 計算したコンフォーマーの数: 30 / 登録したコンフォーマーの数: 28 |