+Open data
-Basic information
Entry | Database: PDB / ID: 1qcb | ||||||
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Title | ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER | ||||||
Components | PROTEIN (HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER) | ||||||
Keywords | ENTEROTOXIN | ||||||
Function / homology | Function and homology information perturbation of signal transduction in another organism / host cell membrane / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Merritt, E.A. / Hol, W.G.J. | ||||||
Citation | Journal: To be Published Title: Crystal Structures of the B-Pentamer from E. coli Enterotoxin LT-IIb Authors: Merritt, E.A. / van den Akker, F. / Connell, T.D. / Holmes, R.K. / Hol, W.G.J. #1: Journal: Structure / Year: 1996 Title: Crystal Structure of a New Heat-Labile Enterotoxin, LT-IIb Authors: Van Den Akker, F. / Sarfaty, S. / Twiddy, E.M. / Connell, T.D. / Holmes, R.K. / Hol, W.G.J. #2: Journal: J.Bacteriol. / Year: 1989 Title: Cloning, Nucleotide Sequence, and Hybridization Studies of the Type IIb Heat- Labile Enterotoxin Gene of Escherichia coli Authors: Pickett, C.L. / Twiddy, E.M. / Coker, C. / Holmes, R.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qcb.cif.gz | 101.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qcb.ent.gz | 83.9 KB | Display | PDB format |
PDBx/mmJSON format | 1qcb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1qcb_validation.pdf.gz | 455.8 KB | Display | wwPDB validaton report |
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Full document | 1qcb_full_validation.pdf.gz | 466.2 KB | Display | |
Data in XML | 1qcb_validation.xml.gz | 21.6 KB | Display | |
Data in CIF | 1qcb_validation.cif.gz | 30.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qc/1qcb ftp://data.pdbj.org/pub/pdb/validation_reports/qc/1qcb | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10778.190 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: HB101 / Plasmid: BLUESCRIPT-KS VECTOR / Production host: Escherichia coli (E. coli) / References: UniProt: P43529 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.8 % |
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 8 / Details: VAPOR DIFFUSION, SITTING DROP, pH 8.0 |
-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 22778 / % possible obs: 83 % / Observed criterion σ(F): 0 / Redundancy: 4 % / Rmerge(I) obs: 0.066 |
Reflection shell | Resolution: 2.2→2.25 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.207 / % possible all: 29 |
-Processing
Software |
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Refinement | Resolution: 2.2→20 Å / Data cutoff low absF: 0.1 / Cross valid method: FREE R / σ(F): 1 / Stereochemistry target values: ENGH AND HUBER / Details: BULK SOLVENT MODELING. METHOD USED : BABINET
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Solvent computation | Solvent model: BABINET | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35 Å2
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Refine analyze | Occupancy sum non hydrogen: 3938 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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