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Open data
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Basic information
Entry | Database: PDB / ID: 1qb5 | ||||||
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Title | ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER | ||||||
![]() | PROTEIN (HEAT LABILE ENTEROTOXIN TYPE IIB B-PENTAMER) | ||||||
![]() | ENTEROTOXIN | ||||||
Function / homology | ![]() perturbation of signal transduction in another organism / host cell membrane / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Merritt, E.A. / Hol, W.G.J. | ||||||
![]() | ![]() Title: Crystal Structures of the B-Pentamer from E. coli Enterotoxin LT-IIb Authors: Merritt, E.A. / Van den Akker, F. / Connell, T.D. / Holmes, R.K. / Hol, W.G.J. #1: ![]() Title: Crystal Structure of a New Heat-Labile Enterotoxin, LT-IIb Authors: Van Den Akker, F. / Sarfaty, S. / Twiddy, E.M. / Connell, T.D. / Holmes, R.K. / Hol, W.G.J. #2: ![]() Title: Cloning, Nucleotide Sequence, and Hybridization Studies of the Type IIb Heat- Labile Enterotoxin Gene of Escherichia coli Authors: Pickett, C.L. / Twiddy, E.M. / Coker, C. / Holmes, R.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.6 KB | Display | ![]() |
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PDB format | ![]() | 83.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.1 KB | Display | ![]() |
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Full document | ![]() | 447.7 KB | Display | |
Data in XML | ![]() | 21.3 KB | Display | |
Data in CIF | ![]() | 29.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10778.190 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.57 Å3/Da / Density % sol: 21.4 % |
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Crystal grow | Method: vapor diffusion, sitting drop / pH: 5.5 / Details: VAPOR DIFFUSION, SITTING DROP, pH 5.5 |
-Data collection
Diffraction | Mean temperature: 275 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 36737 / Num. obs: 36737 / % possible obs: 94 % / Rmerge(I) obs: 0.101 / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.335 / Mean I/σ(I) obs: 5.4 / % possible all: 92 |
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Processing
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Refinement | Resolution: 1.9→10 Å / Num. parameters: 1576 / Num. restraintsaints: 3339 / Cross valid method: FREE R / σ(F): 0 Details: ANISOTROPIC SCALING APPLIED BY THE METHOD OF PARKIN, MOEZZI & HOPE, J.APPL.CRYST.28(1995)53-56
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Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-228 | |||||||||||||||||||||||||||||||||
Refine analyze | Occupancy sum hydrogen: 3675 / Occupancy sum non hydrogen: 3938 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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