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- PDB-1q9f: NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES -
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Open data
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Basic information
Entry | Database: PDB / ID: 1q9f | ||||||
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Title | NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES | ||||||
![]() | Outer membrane protein X | ||||||
![]() | MEMBRANE PROTEIN / OMPX / TROSY / DHPC / DETERGENTS / LIPIDS / MICELLES | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS (CANDID) | ||||||
![]() | Fernandez, C. / Hilty, C. / Wider, G. / Guntert, P. / Wuthrich, K. | ||||||
![]() | ![]() Title: NMR structure of the integral membrane protein OmpX. Authors: Fernandez, C. / Hilty, C. / Wider, G. / Guntert, P. / Wuthrich, K. #1: ![]() Title: Side chain NMR assignments in the membrane protein OmpX reconstituted in DHPC micelles Authors: Hilty, C. / Fernandez, C. / Wider, G. / Wuthrich, K. #2: ![]() Title: Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles Authors: Hilty, C. / Wider, G. / Fernandez, C. / Wuthrich, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 870.2 KB | Display | ![]() |
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PDB format | ![]() | 730.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 358.8 KB | Display | ![]() |
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Full document | ![]() | 487.1 KB | Display | |
Data in XML | ![]() | 48 KB | Display | |
Data in CIF | ![]() | 72.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 16371.768 Da / Num. of mol.: 1 / Mutation: HIS100A to ASN100A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 2MM OMPX U-15N,13C,2H; 2MM OMPX U-15N,13C,2H/L,V,ID1- 13CH3; 2MM OMPX U-15N,10%-13C |
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Sample conditions | Ionic strength: 20mM SODIUM PHOSPHATE, 100mM NACL / pH: 6.5 / Pressure: AMBIENT / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: TORSION ANGLE DYNAMICS (CANDID) / Software ordinal: 1 Details: AUTOMATIC IDENTIFICATION OF HYDROGEN BOND CONSTRAINTS | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 400 / Conformers submitted total number: 20 |