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Open data
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Basic information
Entry | Database: PDB / ID: 1px3 | ||||||
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Title | E. COLI (LACZ) BETA-GALACTOSIDASE (G794A) | ||||||
![]() | beta-galactosidase | ||||||
![]() | HYDROLASE / loop conformation | ||||||
Function / homology | ![]() alkali metal ion binding / lactose catabolic process / beta-galactosidase complex / beta-galactosidase / beta-galactosidase activity / carbohydrate binding / magnesium ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Juers, D.H. / Hakda, S. / Matthews, B.W. / Huber, R.E. | ||||||
![]() | ![]() Title: Structural Basis for the Altered Activity of Gly794 Variants of Escherichia coli Beta-Galactosidase Authors: Juers, D.H. / Hakda, S. / Matthews, B.W. / Huber, R.E. | ||||||
History |
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Remark 600 | HETEROGEN EACH HETEROATOM HAS BEEN ASSIGNED A CHAIN ID AND RESIDUE NUMBER BASED ON AN EQUIVALENCY ...HETEROGEN EACH HETEROATOM HAS BEEN ASSIGNED A CHAIN ID AND RESIDUE NUMBER BASED ON AN EQUIVALENCY TO HETEROATOMS IN PDB ENTRY 1DP0 (NATIVE BETA-GALACTOSIDASE). THE NUMBERING IS AS FOLLOWS: 2001-2002 LIGAND IPT, ISOPROPYL-1-BETA-D-THIOGALACTOSIDE 3001-3999 IONS (MG++ AND NA+) 4001-7999 WATER MOLECULES THOSE WATER MOLECULES WITHOUT AN EQUIVALENT IN 1DP0 START AT 7500 8401-8799 DMS |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 924.8 KB | Display | ![]() |
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PDB format | ![]() | 731.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 537.5 KB | Display | ![]() |
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Full document | ![]() | 693.5 KB | Display | |
Data in XML | ![]() | 191.2 KB | Display | |
Data in CIF | ![]() | 285.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1px4C ![]() 1dp0S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 116520.281 Da / Num. of mol.: 4 / Mutation: G794A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-NA / #4: Chemical | ChemComp-DMS / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.77 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG 8000, NACL, MGCL2, DTT, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 1, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→21.7 Å / Num. obs: 610095 / % possible obs: 92.1 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Biso Wilson estimate: 16 Å2 / Rmerge(I) obs: 0.048 / Net I/σ(I): 22.6 |
Reflection shell | Resolution: 1.6→1.63 Å / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 3.3 / % possible all: 81.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1dp0 Resolution: 1.6→21.7 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: TNT
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Displacement parameters | Biso mean: 0.2 Å2
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Refinement step | Cycle: LAST / Resolution: 1.6→21.7 Å
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Refine LS restraints |
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