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Open data
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Basic information
Entry | Database: PDB / ID: 1pkq | ||||||
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Title | Myelin Oligodendrocyte Glycoprotein-(8-18C5) Fab-complex | ||||||
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![]() | IMMUNE SYSTEM / autoantibody / multiple sclerosis / EAE | ||||||
Function / homology | ![]() response to folic acid / response to lipid / regulation of cytokine production / T cell receptor signaling pathway / cell adhesion / external side of plasma membrane / signaling receptor binding / cell surface Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Breithaupt, C. / Schubart, A. / Zander, H. / Skerra, A. / Huber, R. / Linington, C. / Jacob, U. | ||||||
![]() | ![]() Title: Structural insights into the antigenicity of myelin oligodendrocyte glycoprotein Authors: Breithaupt, C. / Schubart, A. / Zander, H. / Skerra, A. / Huber, R. / Linington, C. / Jacob, U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 219.9 KB | Display | ![]() |
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PDB format | ![]() | 180.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 482.6 KB | Display | ![]() |
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Full document | ![]() | 539.2 KB | Display | |
Data in XML | ![]() | 46.7 KB | Display | |
Data in CIF | ![]() | 64 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 26154.316 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 26683.082 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein | Mass: 15928.754 Da / Num. of mol.: 2 / Fragment: N-terminal Ig domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.55 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: Tris/HCl, MOPS, NaCl, PEG8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Nov 10, 2002 |
Radiation | Monochromator: Si(111) double crystal, non dispersive / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 3→18 Å / Num. obs: 22442 / % possible obs: 86.7 % / Redundancy: 2.3 % / Biso Wilson estimate: 69.1 Å2 / Rmerge(I) obs: 0.094 / Net I/σ(I): 5.3 |
Reflection shell | Resolution: 3→3.09 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.351 / Mean I/σ(I) obs: 2 / Num. unique all: 1590 / % possible all: 73 |
Reflection | *PLUS Num. obs: 22490 |
Reflection shell | *PLUS Highest resolution: 3 Å / Lowest resolution: 3.1 Å / % possible obs: 73 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 33.6 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→18 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.05 Å /
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Refinement | *PLUS Rfactor Rwork: 0.25 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |