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- PDB-1pfv: METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH DI... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1pfv | ||||||
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Title | METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH DIFLUOROMETHIONINE | ||||||
![]() | Methionyl-tRNA synthetase | ||||||
![]() | LIGASE / Rossmann fold | ||||||
Function / homology | ![]() methionine-tRNA ligase / methionine-tRNA ligase activity / methionyl-tRNA aminoacylation / aminoacyl-tRNA synthetase multienzyme complex / tRNA binding / protein homodimerization activity / zinc ion binding / ATP binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Crepin, T. / Schmitt, E. / Mechulam, Y. / Sampson, P.B. / Vaughan, M.D. / Honek, J.F. / Blanquet, S. | ||||||
![]() | ![]() Title: Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase. Authors: Crepin, T. / Schmitt, E. / Mechulam, Y. / Sampson, P.B. / Vaughan, M.D. / Honek, J.F. / Blanquet, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.6 KB | Display | ![]() |
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PDB format | ![]() | 101.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 380.7 KB | Display | ![]() |
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Full document | ![]() | 387.9 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 21.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1p7pC ![]() 1pfuC ![]() 1pfwC ![]() 1pfyC ![]() 1pg0C ![]() 1pg2C ![]() 1qqtS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 62958.102 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-551 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-2FM / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.92 % |
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Crystal grow | Temperature: 280 K / Method: vapor diffusion / pH: 7 Details: AMMONIUM CITRATE, POTASSIUM PHOSPHATE, pH 7, VAPOR DIFFUSION, temperature 280K |
Crystal grow | *PLUS Method: unknown / Details: Mechulam, Y., (1999) J. Mol. Biol., 294, 1287. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 13, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→74 Å / Num. all: 63519 / Num. obs: 63519 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 15.7 Å2 / Rsym value: 0.051 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 1.7→1.74 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 3.8 / Num. unique all: 4755 / Rsym value: 0.19 / % possible all: 99.8 |
Reflection | *PLUS Num. obs: 63973 / Rmerge(I) obs: 0.051 |
Reflection shell | *PLUS % possible obs: 99.8 % / Rmerge(I) obs: 0.19 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1QQT Resolution: 1.7→74 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.7→74 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.71 Å
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rfree: 0.204 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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