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Open data
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Basic information
Entry | Database: PDB / ID: 1pdz | ||||||
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Title | X-RAY STRUCTURE AND CATALYTIC MECHANISM OF LOBSTER ENOLASE | ||||||
![]() | ENOLASE | ||||||
![]() | LYASE (CARBON-OXYGEN) | ||||||
Function / homology | ![]() phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / glycolytic process / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Janin, J. / Duquerroy, S. / Camus, C. / Le Bras, G. | ||||||
![]() | ![]() Title: X-ray structure and catalytic mechanism of lobster enolase. Authors: Duquerroy, S. / Camus, C. / Janin, J. #1: ![]() Title: Phasing with Mercury at 1 Angstrom Wavelength Authors: Dumas, C. / Duquerroy, S. / Janin, J. #2: ![]() Title: Lobster Enolase Crystallized by Serendipity Authors: Duquerroy, S. / Le Bras, G. / Janin, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97 KB | Display | ![]() |
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PDB format | ![]() | 74.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 379.1 KB | Display | ![]() |
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Full document | ![]() | 389 KB | Display | |
Data in XML | ![]() | 11.1 KB | Display | |
Data in CIF | ![]() | 16.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 47124.559 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Chemical | ChemComp-PGA / |
#3: Chemical | ChemComp-MN / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.42 % | |||||||||||||||
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Crystal | *PLUS Density % sol: 57 % | |||||||||||||||
Crystal grow | *PLUS Temperature: 16 ℃ / pH: 7.6 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() |
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Detector | Detector: IMAGE PLATE / Date: Feb 1, 1992 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 27950 / % possible obs: 91.4 % / Redundancy: 4.1 % / Rmerge(I) obs: 0.077 |
Reflection | *PLUS Num. measured all: 97010 / Rmerge(I) obs: 0.077 |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.3 Å / % possible obs: 43.1 % / Rmerge(I) obs: 0.393 |
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Processing
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Refinement | Resolution: 2.2→8 Å / σ(F): 2 /
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Displacement parameters | Biso mean: 37.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Rfactor obs: 0.217 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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