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Yorodumi- PDB-1pav: SOLUTION NMR STRUCTURE OF HYPOTHETICAL PROTEIN TA1414 OF THERMOPL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1pav | ||||||
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Title | SOLUTION NMR STRUCTURE OF HYPOTHETICAL PROTEIN TA1414 OF THERMOPLASMA ACIDOPHILUM | ||||||
Components | Hypothetical protein Ta1170/Ta1414 | ||||||
Keywords | STRUCTURAL GENOMICS / thermoplasma acidophilum / fast NMR / semiautomated analysis | ||||||
Function / homology | TusA-like domain / Uncharacterized protein family UPF0033 signature. / TusA-like domain / Sulfurtransferase TusA / TusA-like domain superfamily / Translation Initiation Factor IF3 / 2-Layer Sandwich / Alpha Beta / Putative sulfur carrier protein Ta1170/Ta1414 Function and homology information | ||||||
Biological species | Thermoplasma acidophilum (acidophilic) | ||||||
Method | SOLUTION NMR / Initial manual assignment of unambiguous NOEs. Further assignment of spectra by NOAH routine at DYANA. | ||||||
Authors | Monleon, D. / Yee, A. / Liu, C.S. / Arrowsmith, C. / Celda, B. | ||||||
Citation | Journal: J.Biomol.Nmr / Year: 2004 Title: Solution structure of hypothetical protein TA1414 from Thermoplasma acidophilum. Authors: Monleon, D. / Yee, A. / Liu, C.S. / Arrowsmith, C. / Celda, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pav.cif.gz | 240.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pav.ent.gz | 184.5 KB | Display | PDB format |
PDBx/mmJSON format | 1pav.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1pav_validation.pdf.gz | 340 KB | Display | wwPDB validaton report |
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Full document | 1pav_full_validation.pdf.gz | 576 KB | Display | |
Data in XML | 1pav_validation.xml.gz | 115.2 KB | Display | |
Data in CIF | 1pav_validation.cif.gz | 142.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pa/1pav ftp://data.pdbj.org/pub/pdb/validation_reports/pa/1pav | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8695.097 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermoplasma acidophilum (acidophilic) / Gene: TA1170 AND TA1414 / Plasmid: PET15B / Production host: Escherichia coli (E. coli) / References: UniProt: Q9HI35 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: structural genomics sample |
-Sample preparation
Details | Contents: 2mM ta1414 U-15N,13C, 50mM phosphate buffer, pH 6.5 Solvent system: 95% H2O/5% D2O |
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Sample conditions | pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
-Processing
NMR software |
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Refinement | Method: Initial manual assignment of unambiguous NOEs. Further assignment of spectra by NOAH routine at DYANA. Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 100 / Conformers submitted total number: 10 |