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- PDB-1ou8: structure of an AAA+ protease delivery protein in complex with a ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ou8 | ||||||
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Title | structure of an AAA+ protease delivery protein in complex with a peptide degradation tag | ||||||
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![]() | TRANSPORT PROTEIN / peptide-binding pocket / protein-peptide complex / homodimer | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Levchenko, I. / Grant, R.A. / Wah, D.A. / Sauer, R.T. / Baker, T.A. | ||||||
![]() | ![]() Title: Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag Authors: Levchenko, I. / Grant, R.A. / Wah, D.A. / Sauer, R.T. / Baker, T.A. #1: ![]() Title: a specificity-enhancing factor for the ClpXP degradation machine Authors: Levchenko, I. / Siedel, M. / Sauer, R.T. / Baker, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63 KB | Display | ![]() |
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PDB format | ![]() | 45.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.7 KB | Display | ![]() |
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Full document | ![]() | 451.3 KB | Display | |
Data in XML | ![]() | 12.6 KB | Display | |
Data in CIF | ![]() | 17.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ou9SC ![]() 1oulC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12640.204 Da / Num. of mol.: 2 / Fragment: residues 1-111 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein/peptide | Mass: 1205.196 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: THE PEPTIDE WAS CHEMICALLY SYNTHESIZED. / Source: (synth.) synthetic construct (others) #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.42 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.7 Details: sodium citrate, sodium cacodylate, sodium thiocyanate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 5.6 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Sep 30, 2002 |
Radiation | Monochromator: KOHZU double crystal monochromator sagitally focused second crystal Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92108 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. all: 38849 / Num. obs: 38849 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10.8 % / Biso Wilson estimate: 17.8 Å2 / Rsym value: 0.107 |
Reflection shell | Resolution: 1.6→1.7 Å / Rsym value: 0.634 / % possible all: 100 |
Reflection | *PLUS Num. measured all: 418776 / Rmerge(I) obs: 0.107 |
Reflection shell | *PLUS % possible obs: 100 % / Rmerge(I) obs: 0.634 / Mean I/σ(I) obs: 3.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1OU9 Resolution: 1.6→14.98 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 226623.29 / Data cutoff high rms absF: 226623.29 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.8539 Å2 / ksol: 0.411845 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.6→14.98 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.6→1.7 Å / Rfactor Rfree error: 0.01 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Version: 1.1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.6 Å / % reflection Rfree: 10 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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