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Open data
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Basic information
Entry | Database: PDB / ID: 1otk | ||||||
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Title | Structural Genomics, Protein paaC | ||||||
![]() | Phenylacetic acid degradation protein paaC | ||||||
![]() | OXIDOREDUCTASE / STRUCTURAL GENOMICS / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | ![]() phenylacetyl-CoA 1,2-epoxidase complex / phenylacetate catabolic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, R. / Joachimiak, A. / Edwards, A. / Savchenko, A. / Skarina, T. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: The 2 A crystal structure of protein paaC from E. Coli Authors: Zhang, R. / Joachimiak, A. / Edwards, A. / Savchenko, A. / Skarina, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.6 KB | Display | ![]() |
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PDB format | ![]() | 87.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430 KB | Display | ![]() |
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Full document | ![]() | 434 KB | Display | |
Data in XML | ![]() | 22.2 KB | Display | |
Data in CIF | ![]() | 32.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | This protein exists as dimer which consists of molA and molB. |
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Components
#1: Protein | Mass: 27969.404 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.22 Å3/Da / Density % sol: 61.78 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 11% iso-Prop., 0.1 M Nacacod., 0.2M NaCitr., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: SBC-2 / Detector: CCD / Date: Mar 17, 2003 / Details: Si 111 Channel |
Radiation | Monochromator: Si 111 Channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 49792 / Num. obs: 49742 / % possible obs: 99.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 10.76 % / Biso Wilson estimate: 12.7 Å2 / Rmerge(I) obs: 0.113 / Net I/σ(I): 33.1 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.52 / Mean I/σ(I) obs: 5.84 / Num. unique all: 4898 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Details: The number of reflections for refinement includes Freidel pairs.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 50.7366 Å2 / ksol: 0.34617 e/Å3 | ||||||||||||||||||||
Displacement parameters | Biso mean: 22.9 Å2
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Refine analyze | Luzzati coordinate error free: 0.25 Å / Luzzati sigma a free: 0.18 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→46.82 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.009 / Total num. of bins used: 6
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Xplor file |
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