+Open data
-Basic information
Entry | Database: PDB / ID: 1osi | ||||||
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Title | STRUCTURE OF 3-ISOPROPYLMALATE DEHYDROGENASE | ||||||
Components | 3-ISOPROPYLMALATE DEHYDROGENASE | ||||||
Keywords | OXIDOREDUCTASE / DEHYDROGENASE | ||||||
Function / homology | Function and homology information 3-isopropylmalate dehydrogenase / 3-isopropylmalate dehydrogenase activity / L-leucine biosynthetic process / NAD binding / magnesium ion binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3 Å | ||||||
Authors | Qu, C. / Akanuma, S. / Moriyama, H. / Tanaka, N. / Oshima, T. | ||||||
Citation | Journal: Protein Eng. / Year: 1997 Title: A mutation at the interface between domains causes rearrangement of domains in 3-isopropylmalate dehydrogenase. Authors: Qu, C. / Akanuma, S. / Moriyama, H. / Tanaka, N. / Oshima, T. #1: Journal: J.Biochem.(Tokyo) / Year: 1990 Title: Purification, Catalytic Properties, and Thermal Stability of Threo-Ds-3-Isopropylmalate Dehydrogenase Coded by Leub Gene from an Extreme Thermophile, Thermus Thermophilus Strain Hb8 Authors: Yamada, T. / Akutsu, N. / Miyazaki, K. / Kakinuma, K. / Yoshida, M. / Oshima, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1osi.cif.gz | 299.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1osi.ent.gz | 247.8 KB | Display | PDB format |
PDBx/mmJSON format | 1osi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1osi_validation.pdf.gz | 431.8 KB | Display | wwPDB validaton report |
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Full document | 1osi_full_validation.pdf.gz | 462 KB | Display | |
Data in XML | 1osi_validation.xml.gz | 47.5 KB | Display | |
Data in CIF | 1osi_validation.cif.gz | 65.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/os/1osi ftp://data.pdbj.org/pub/pdb/validation_reports/os/1osi | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | THE ASYMMETRIC UNIT CONTAINS TWO FUNCTIONAL DIMERS, FORMED BY THE SUBUNITS A AND C, B AND D. |
-Components
#1: Protein | Mass: 36825.160 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Production host: unidentified (others) References: UniProt: Q5SIY4, 3-isopropylmalate dehydrogenase |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 58.2 % | |||||||||||||||||||||||||
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Crystal grow | pH: 4.8 Details: THE STRUCTURE SOLVED USING CRYSTALS GROWN FROM AMMONIUM SULFATE WAS DEPOSITED AS 1IPD AT PDB. THE CRYSTALLIZATION CONDITION IN THIS STRUCTURE WAS SIMILAR AS THE MUTANT A172L., pH 4.8 | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.6 / Method: unknown | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jul 15, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 27930 / % possible obs: 93.8 % / Observed criterion σ(I): 1 / Redundancy: 0.927 % / Rmerge(I) obs: 0.054 |
-Processing
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Refinement | Resolution: 3→6 Å / σ(F): 2 /
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Displacement parameters | Biso mean: 26.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→6 Å
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Refine LS restraints |
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