分子量: 2253.781 Da / 分子数: 1 / 断片: C-terminal fragment / 由来タイプ: 合成 詳細: THE Peptide WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE OF THE Peptide IS NATURALLY FOUND IN DROSOPHILA MELANOGASTER (fruit fly). The peptide was obtained from Neosystem Inc. and used without further purification. 参照: UniProt: P02833
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
1
2
1
2D TOCSY
NMR実験の詳細
Text: Structure was determined by standard 2D homonuclear techniques
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試料調製
詳細
内容: 3mM penetratin, 50 mM KCl; 15% w/v (DMPC+DMPG)/DHPC bicellar solution with q=0.5 and 5% D2O 溶媒系: 15% w/v (DMPC+DMPG)/DHPC bicellar solution with q=0.5 and 5% D2O
手法: distance geometry / ソフトェア番号: 1 詳細: A total of 129 distance constraints where identified, mostly sequential and medium range NOEs (40 intra-residue, 54 sequential and 35 medium range NOEs)
代表構造
選択基準: closest to the average,lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 60 / 登録したコンフォーマーの数: 20