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- PDB-1o82: X-RAY STRUCTURE OF BACTERIOCIN AS-48 AT PH 4.5. SULPHATE BOUND FORM -
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Open data
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Basic information
Entry | Database: PDB / ID: 1o82 | ||||||
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Title | X-RAY STRUCTURE OF BACTERIOCIN AS-48 AT PH 4.5. SULPHATE BOUND FORM | ||||||
![]() | PEPTIDE ANTIBIOTIC AS-48 | ||||||
![]() | PEPTIDE ANTIBIOTIC / BACTERIOCIN / CATIONIC ANTIBACTERIAL PEPTIDES / MEMBRANE PERMEABILIZATION / PROTEIN CRYSTALLOGRAPHY / CYCLIC POLYPEPTIDE / PROTEIN MEMBRANE INTERACTION | ||||||
Function / homology | ![]() killing of cells of another organism / defense response to bacterium / extracellular region / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sanchez-Barrena, M.J. / Martinez-Ripoll, M. / Galvez, A. / Martinez-Bueno, M. / Maqueda, M. / Cruz, V. / Albert, A. | ||||||
![]() | ![]() Title: Structure of Bacteriocin as-48: From Soluble State to Membrane Bound State Authors: Sanchez-Barrena, M.J. / Martinez-Ripoll, M. / Galvez, A. / Valdivia, E. / Maqueda, M. / Cruz, V. / Albert, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.7 KB | Display | ![]() |
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PDB format | ![]() | 51.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 7177.538 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Details: PEPTIDE LINK BETWEEN RESIDUES 1 AND 70 / Source: (natural) ![]() ![]() #2: Chemical | ChemComp-GOL / | #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.42 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 Details: 0.2 M AMMONIUM SULFATE, 0.1 SODIUM ACETATE TRIHYDRATE PH 4.5, 12% W/V POLYETHYLENE GLYCOL 4000AS-48 10 MG/ML | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.46→25.25 Å / Num. obs: 54856 / % possible obs: 94.6 % / Redundancy: 4 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 6.1 |
Reflection shell | Resolution: 1.46→1.54 Å / Redundancy: 3 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 2.4 / % possible all: 94.6 |
Reflection | *PLUS Highest resolution: 1.46 Å / Lowest resolution: 25.24 Å / Redundancy: 4 % / Rmerge(I) obs: 0.064 |
Reflection shell | *PLUS % possible obs: 94.6 % / Redundancy: 3 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 2.4 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
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Displacement parameters | Biso mean: 19.601 Å2
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Refinement step | Cycle: LAST / Resolution: 1.46→15 Å
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Refinement | *PLUS Lowest resolution: 15 Å / Rfactor Rfree: 0.2235 / Rfactor Rwork: 0.226 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 1.46 Å / Lowest resolution: 1.54 Å / Rfactor Rfree: 0.34 / Rfactor Rwork: 0.29 |