STRUCTURES WITH ACCEPTABLE COVALEN GEOMETRY, STRUCTURES WITH FAVORABL NON-BOND ENERGY
Representative
Model #1
-
Components
#1: Protein
PRE-MRNAPROCESSINGPROTEINPRP40 / PRP40
Mass: 8897.083 Da / Num. of mol.: 1 / Fragment: WW DOMAIN PAIR, RESIDUES 1-75 Source method: isolated from a genetically manipulated source Details: WW DOMAIN PAIR OF SC. PRE-MRNA PROCESSING PROTEIN (PRP) 40 Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Description: N-TERMINAL HIS6-TAG AND TEV CLEAVAGE SITE / Plasmid: PET24D / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P33203
Compound details
REQUIRED FOR THE FIRST STEP OF PRE-MRNA SPLICING. THIS PROTEIN IS ASSOCIATED WITH SNRNP U1.
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
13C-NOESY
1
2
1
15N-NOESY
1
3
1
S3CT
1
4
1
HNCG
1
5
1
HNHA-J
NMR details
Text: THE STRUCTURE WAS DETERMINED USING STANDARD TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C,15N-LABELED PRP40 1-75. RESIDUAL DIPOLAR COUPLINGS WERE MEASURED TO INCREASE THE CONVERGENCE OF THE SET OF STUCTURES CALCULATED.
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Sample preparation
Details
Solution-ID
Contents
1
1.5 MM 15N- OR 13C,15N
2
20MMPHOSPHATEBUFFERPH6.3
3
30MMNACL
4
0.03% (W/V) NAN3
Sample conditions
Ionic strength: 20MM NA PHOSPHATE, 30MM NACL / pH: 6.3 / Pressure: 1 atm / Temperature: 295 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
500
1
Bruker DRX
Bruker
DRX
600
2
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Processing
NMR software
Name
Version
Developer
Classification
ARIA
NILGES, M.
refinement
XEASY
structuresolution
NMRView
structuresolution
PIPP/ CAPP
CAPP
structuresolution
ARIA
structuresolution
CNS
structuresolution
Refinement
Method: AMBIGUOUS DISTANCE RESTRAINTS SIMULATED ANNEALING WIT TORSION ANGLE DYNAMICS AS IMPLEMENTED IN ARIA1.0 Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensemble
Conformer selection criteria: STRUCTURES WITH ACCEPTABLE COVALEN GEOMETRY, STRUCTURES WITH FAVORABL NON-BOND ENERGY Conformers calculated total number: 20 / Conformers submitted total number: 7
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