+Open data
-Basic information
Entry | Database: PDB / ID: 1nsh | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of Rabbit apo-S100A11 (19 models) | ||||||
Components | Calgizzarin | ||||||
Keywords | METAL BINDING PROTEIN / calcium-binding protein / EF hand / helix-loop-helix / S100 / Annexin | ||||||
Function / homology | Function and homology information calcium-dependent protein binding / regulation of cell population proliferation / calcium ion binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Oryctolagus cuniculus (rabbit) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Dempsey, A.C. / Walsh, M.P. / Shaw, G.S. | ||||||
Citation | Journal: Structure / Year: 2003 Title: Unmasking the Annexin I Interaction from the structure of apo-S100A11 Authors: Dempsey, A.C. / Walsh, M.P. / Shaw, G.S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1nsh.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1nsh.ent.gz | 978.5 KB | Display | PDB format |
PDBx/mmJSON format | 1nsh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1nsh_validation.pdf.gz | 356.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1nsh_full_validation.pdf.gz | 674.5 KB | Display | |
Data in XML | 1nsh_validation.xml.gz | 101.8 KB | Display | |
Data in CIF | 1nsh_validation.cif.gz | 136.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ns/1nsh ftp://data.pdbj.org/pub/pdb/validation_reports/ns/1nsh | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 11312.939 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Oryctolagus cuniculus (rabbit) / Gene: S100A11 OR S100C OR PCALG / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P24480 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample conditions |
| |||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 Details: Structures were calculated using 2964 NOE derived distance restraints: 1336 intraresidue, 658 sequential, 594 short range, 264 long range and 112 intermonomer. There were 64 hydrogen bond ...Details: Structures were calculated using 2964 NOE derived distance restraints: 1336 intraresidue, 658 sequential, 594 short range, 264 long range and 112 intermonomer. There were 64 hydrogen bond distance restraints and 192 dihedral restraints included in calculations. | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 19 |