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Open data
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Basic information
Entry | Database: PDB / ID: 1npo | ||||||
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Title | BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN | ||||||
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![]() | COMPLEX (HORMONE TRANSPORT/HORMONE) / COMPLEX (HORMONE TRANSPORT-HORMONE) / HYPOTHALAMUS / COMPLEX (HORMONE TRANSPORT-HORMONE) complex | ||||||
Function / homology | ![]() neurohypophyseal hormone activity / V1A vasopressin receptor binding / neuropeptide hormone activity / vasoconstriction / secretory granule / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Rose, J.P. / Wang, B.-C. | ||||||
![]() | ![]() Title: Crystal structure of the neurophysin-oxytocin complex. Authors: Rose, J.P. / Wu, C.K. / Hsiao, C.D. / Breslow, E. / Wang, B.C. #1: ![]() Title: Crystal Structure of a Bovine Neurophysin II Dipeptide Complex at 2.8 A Determined from the Single-Wavelength Anomalous Scattering Signal of an Incorporated Iodine Atom Authors: Chen, L.Q. / Rose, J.P. / Breslow, E. / Yang, D. / Chang, W.R. / Furey Junior, W.F. / Sax, M. / Wang, B.C. #2: ![]() Title: Crystals of Modified Bovine Neurophysin II Authors: Rose, J.P. / Yang, D. / Yoo, C.S. / Sax, M. / Breslow, E. / Wang, B.C. #3: ![]() Title: Crystals of a Bovine Neurophysin II-Dipeptide Amide Complex Authors: Yoo, C.S. / Wang, B.C. / Sax, M. / Breslow, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.5 KB | Display | ![]() |
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PDB format | ![]() | 31.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9890.252 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
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#2: Protein/peptide | Mass: 1010.188 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source #3: Protein | | Mass: 9832.150 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 58 % / Description: DATA WAS COLLECTED IN 2 ORIENTATIONS. | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.8 / Details: pH 6.8 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 K / pH: 6 / Method: batch method | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS-NICOLET X100 / Detector: AREA DETECTOR / Date: Jun 6, 1990 / Details: SUPPER MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 3 Å / Num. obs: 5924 / % possible obs: 99.99 % / Redundancy: 6.4 % / Biso Wilson estimate: 29.17 Å2 / Rmerge(I) obs: 0.056 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 3→3.18 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.253 / Mean I/σ(I) obs: 1.7 / % possible all: 100 |
Reflection | *PLUS Num. all: 5928 / Num. measured all: 38324 |
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Processing
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Refinement | Resolution: 3→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 30.13 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.3 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3→3.12 Å
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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