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Open data
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Basic information
Entry | Database: PDB / ID: 1noq | ||||||||||||||||||
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Title | e-motif structure | ||||||||||||||||||
![]() | 5'-D(*![]() DNA / e-Motif / (ccg)2 DNA duplex | Function / homology | DNA | ![]() Method | SOLUTION NMR / distance geometry, restraint molecular dynamics | ![]() Zheng, M. / Huang, X. / Smith, G.K. / Yang, X. / Gao, X. | ![]() ![]() Title: Genetically unstable CXG repeats are structurally dynamic and have a high propensity for folding. An NMR and UV spectroscopic study. Authors: Zheng, M. / Huang, X. / Smith, G.K. / Yang, X. / Gao, X. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 50.5 KB | Display | ![]() |
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PDB format | ![]() | 31.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 305.7 KB | Display | ![]() |
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Full document | ![]() | 336.4 KB | Display | |
Data in XML | ![]() | 6.4 KB | Display | |
Data in CIF | ![]() | 9.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: DNA chain | Mass: 1770.181 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: this sequence is found in CCG and/or CGG triplet repeat expansion in the 5'-untranslated region of FMR1 gene of fragile X syndrome. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques |
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Sample preparation
Details | Contents: 2 mM 5'-d(CCGCCG), 0.1 M NaCl, 10 mM Sodium Phosphate, 0.1 mM EDTA Solvent system: 90 % H20, 10 % D2O |
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Sample conditions | pH: 6.3 / Pressure: ambient / Temperature: 278 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: distance geometry, restraint molecular dynamics / Software ordinal: 1 Details: the structures are determined with a total of 442 restraints: 430 are NOE-derived distance constraints based on ISPA (isolated spin pair approximation) and 12 are dihedral angle restraints ...Details: the structures are determined with a total of 442 restraints: 430 are NOE-derived distance constraints based on ISPA (isolated spin pair approximation) and 12 are dihedral angle restraints from the analyses of the COSY type of spectra. | ||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 6 / Conformers submitted total number: 6 |