[English] 日本語
![](img/lk-miru.gif)
- PDB-1nl4: Crystal Structure of Rat Farnesyl Transferase in Complex With A P... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1nl4 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Rat Farnesyl Transferase in Complex With A Potent Biphenyl Inhibitor | ||||||
![]() |
| ||||||
![]() | TRANSFERASE / Prenyltransferase | ||||||
Function / homology | ![]() Apoptotic cleavage of cellular proteins / Inactivation, recovery and regulation of the phototransduction cascade / RAS processing / protein geranylgeranyltransferase activity / protein geranylgeranyltransferase type I / CAAX-protein geranylgeranyltransferase activity / CAAX-protein geranylgeranyltransferase complex / protein farnesylation / positive regulation of tubulin deacetylation / protein farnesyltransferase ...Apoptotic cleavage of cellular proteins / Inactivation, recovery and regulation of the phototransduction cascade / RAS processing / protein geranylgeranyltransferase activity / protein geranylgeranyltransferase type I / CAAX-protein geranylgeranyltransferase activity / CAAX-protein geranylgeranyltransferase complex / protein farnesylation / positive regulation of tubulin deacetylation / protein farnesyltransferase / protein farnesyltransferase activity / protein farnesyltransferase complex / regulation of fibroblast proliferation / Rab geranylgeranyltransferase activity / protein geranylgeranylation / positive regulation of skeletal muscle acetylcholine-gated channel clustering / negative regulation of nitric-oxide synthase biosynthetic process / farnesyltranstransferase activity / microtubule associated complex / enzyme-linked receptor protein signaling pathway / positive regulation of nitric-oxide synthase biosynthetic process / positive regulation of Rac protein signal transduction / alpha-tubulin binding / : / positive regulation of cell cycle / response to cytokine / wound healing / response to organic cyclic compound / lipid metabolic process / receptor tyrosine kinase binding / positive regulation of fibroblast proliferation / fibroblast proliferation / microtubule binding / cell population proliferation / molecular adaptor activity / negative regulation of cell population proliferation / positive regulation of cell population proliferation / negative regulation of apoptotic process / zinc ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Curtin, M.L. / Florjancic, A.S. / Cohen, J. / Gu, W.-J. / Frost, D.J. / Muchmore, S.W. / Sham, H.L. | ||||||
![]() | ![]() Title: Novel and Selective Imidazole-containing Biphenyl Inhibitors of Protein Farnesyltransferase Authors: Curtin, M.L. / Florjancic, A.S. / Cohen, J. / Gu, W.-J. / Frost, D.J. / Muchmore, S.W. / Sham, H.L. | ||||||
History |
| ||||||
Remark 999 | sequence Author indicates that this represents an inconsequential mutation |
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 139.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 105.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 507.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 546.8 KB | Display | |
Data in XML | ![]() | 21.1 KB | Display | |
Data in CIF | ![]() | 30.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 37634.293 Da / Num. of mol.: 1 / Fragment: residue 55-366 / Mutation: I156T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q04631, Transferases; Transferring alkyl or aryl groups, other than methyl groups |
---|---|
#2: Protein | Mass: 44879.297 Da / Num. of mol.: 1 / Fragment: residue 23-423 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q02293, Transferases; Transferring alkyl or aryl groups, other than methyl groups |
#3: Chemical | ChemComp-ZN / |
#4: Chemical | ChemComp-HFP / |
#5: Chemical | ChemComp-FTL / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.44 Å3/Da / Density % sol: 64.21 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: equilibriate equal volumes of 10mg/ml protein stock solution (with 50mM Tris, 1mM DTT, 1mM NaN3, 5 mM ZnCl2-pH 8.2) and well solution containing 100mM KCl, 100mM Sodium Acetate, at pH 4.8, ...Details: equilibriate equal volumes of 10mg/ml protein stock solution (with 50mM Tris, 1mM DTT, 1mM NaN3, 5 mM ZnCl2-pH 8.2) and well solution containing 100mM KCl, 100mM Sodium Acetate, at pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystal grow | *PLUS Details: Strickland, C.L., (19999) J. Med. Chem., 42, 2125. |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 6, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 26759 / Num. obs: 26759 / % possible obs: 85 % / Observed criterion σ(F): 5 / Observed criterion σ(I): 3 / Rmerge(I) obs: 0.056 / Rsym value: 0.056 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 50 Å / % possible obs: 98 % / Rmerge(I) obs: 0.048 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→50 Å
| |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.8 Å / Rfactor Rfree: 0.321 / Rfactor Rwork: 0.249 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |