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Open data
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Basic information
Entry | Database: PDB / ID: 1mx6 | ||||||
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Title | Structure of p18INK4c (F92N) | ||||||
![]() | Cyclin-dependent kinase 6 inhibitor | ||||||
![]() | cell cycle inhibitor / Ankyrin repeats | ||||||
Function / homology | ![]() negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / regulation of cyclin-dependent protein serine/threonine kinase activity / oligodendrocyte differentiation / regulation of G1/S transition of mitotic cell cycle / negative regulation of stem cell proliferation / stem cell proliferation / Oncogene Induced Senescence / negative regulation of cell growth / Cyclin D associated events in G1 ...negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / regulation of cyclin-dependent protein serine/threonine kinase activity / oligodendrocyte differentiation / regulation of G1/S transition of mitotic cell cycle / negative regulation of stem cell proliferation / stem cell proliferation / Oncogene Induced Senescence / negative regulation of cell growth / Cyclin D associated events in G1 / Senescence-Associated Secretory Phenotype (SASP) / Oxidative Stress Induced Senescence / cell cycle / negative regulation of cell population proliferation / protein kinase binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Marmorstein, R. / Venkataramani, R.N. / MacLachlan, T.K. / Chai, X. / El-Deiry, W.S. | ||||||
![]() | ![]() Title: Structure-based design of p18INK4c proteins with increased thermodynamic stability and cell cycle inhibitory activity Authors: Venkataramani, R.N. / MacLachlan, T.K. / Chai, X. / El-Deiry, W.S. / Marmorstein, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.4 KB | Display | ![]() |
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PDB format | ![]() | 54.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373 KB | Display | ![]() |
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Full document | ![]() | 378.8 KB | Display | |
Data in XML | ![]() | 7.5 KB | Display | |
Data in CIF | ![]() | 12.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mx2C ![]() 1mx4C ![]() 1ihbS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18116.285 Da / Num. of mol.: 2 / Mutation: F92N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.05 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 273 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 5 mg/ml protein, 20 mM Tris (pH 8.5), 0.5 mM DTT, 14 % PEG 6000 and 2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 273K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Details: mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 23674 / Num. obs: 22869 / % possible obs: 96.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.062 / Rsym value: 0.062 / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 2→2.05 Å / Rmerge(I) obs: 0.496 / Mean I/σ(I) obs: 2.7 / Rsym value: 0.496 / % possible all: 91.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1IHB Resolution: 2→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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Xplor file |
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Refinement | *PLUS Rfactor obs: 0.237 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |