[English] 日本語
Yorodumi- PDB-1muc: STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION -
+Open data
-Basic information
Entry | Database: PDB / ID: 1muc | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION | |||||||||
Components | MUCONATE LACTONIZING ENZYME | |||||||||
Keywords | ISOMERASE / MUCONATE LACTONIZING ENZYME | |||||||||
Function / homology | Function and homology information muconate cycloisomerase / chloromuconate cycloisomerase activity / muconate cycloisomerase activity / beta-ketoadipate pathway / amino acid catabolic process / manganese ion binding Similarity search - Function | |||||||||
Biological species | Pseudomonas putida (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.85 Å | |||||||||
Authors | Helin, S. / Kahn, P.C. / Guha, B.H.L. / Mallows, D.J. / Goldman, A. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1995 Title: The refined X-ray structure of muconate lactonizing enzyme from Pseudomonas putida PRS2000 at 1.85 A resolution. Authors: Helin, S. / Kahn, P.C. / Guha, B.L. / Mallows, D.G. / Goldman, A. #1: Journal: J.Mol.Biol. / Year: 1987 Title: Crystal Structure of Muconate Lactonizing Enzyme at 3 A Resolution Authors: Goldman, A. / Ollis, D.L. / Steitz, T.A. #2: Journal: J.Mol.Biol. / Year: 1985 Title: Crystal Structure of Muconate Lactonizing Enzyme at 6.5 A Resolution Authors: Goldman, A. / Ollis, D. / Ngai, K.L. / Steitz, T.A. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1muc.cif.gz | 151 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1muc.ent.gz | 118.6 KB | Display | PDB format |
PDBx/mmJSON format | 1muc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1muc_validation.pdf.gz | 373.7 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1muc_full_validation.pdf.gz | 378.3 KB | Display | |
Data in XML | 1muc_validation.xml.gz | 14.6 KB | Display | |
Data in CIF | 1muc_validation.cif.gz | 24 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mu/1muc ftp://data.pdbj.org/pub/pdb/validation_reports/mu/1muc | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.7537, 0.6572, 0.0035), Vector: |
-Components
#1: Protein | Mass: 40355.172 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Pseudomonas putida (bacteria) / Strain: PRS2000 / References: UniProt: P08310, muconate cycloisomerase #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 4 |
---|
-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 52 % |
---|---|
Crystal | *PLUS |
Crystal grow | *PLUS Method: unknown |
-Data collection
Diffraction source | Wavelength: 1.5418 |
---|---|
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Jan 1, 1985 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 66609 / % possible obs: 87.2 % / Redundancy: 3.2 % / Rmerge(I) obs: 0.093 |
Reflection | *PLUS Highest resolution: 1.85 Å / Lowest resolution: 8 Å / Num. obs: 59010 / Num. measured all: 191642 / Rmerge(I) obs: 0.193 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.85→8 Å / σ(F): 1 Details: FOR RESIDUE ARG A 20 ELECTRON DENSITY WAS VISIBLE ONLY FOR BACKBONE ATOMS.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.85→8 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|