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- PDB-1mnx: The Solution Structure of the Loop E Region of the 5S rRNA from S... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mnx | ||||||
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Title | The Solution Structure of the Loop E Region of the 5S rRNA from Spinach Chloroplasts. | ||||||
![]() | Loop E from 5S rRNA | ||||||
![]() | RNA / Loop E / 5S rRNA | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics, molecular dynamics | ||||||
Model type details | minimized average | ||||||
![]() | Vallurupalli, P. / Moore, P.B. | ||||||
![]() | ![]() Title: The Solution Structure of the Loop E Region of the 5S rRNA from Spinach Chloroplasts Authors: Vallurupalli, P. / Moore, P.B. #1: ![]() Title: The 5S rRNA loop E: Chemical probing and phylogenetic data versus crystal structure Authors: Leontis, N.B. / Westhof, E. #2: ![]() Title: The loop E-loop D region of Escherichia coli 5S rRNA: the solution structure reveals an unusual loop that may be important for binding ribosomal proteins. Authors: Dallas, A. / Moore, P.B. #3: ![]() Title: Metals, Motifs, and Recognition in the Crystal Structure of a 5S rRNA domain Authors: Correll, C. / Freeborn, B. / Moore, P.B. / Steitz, T.A. #4: ![]() Title: Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution Authors: Lu, M. / Steitz, T.A. #5: ![]() Title: The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition Authors: Stoldt, M. / Wohnert, J. / Ohlenschlager, O. / Gorlach, M. / Brown, L.R. #6: ![]() Title: Higher order structure of chloroplastic 5S ribosomal RNA from spinach. Authors: Romby, P. / Westhof, E. / Toukifimpa, R. / Mache, R. / Ebel, J.P. / Ehresmann, C. / Ehresmann, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 350.7 KB | Display | ![]() |
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PDB format | ![]() | 296.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 323.5 KB | Display | ![]() |
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Full document | ![]() | 395.3 KB | Display | |
Data in XML | ![]() | 6.3 KB | Display | |
Data in CIF | ![]() | 12.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: RNA chain | Mass: 13667.229 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was in vitro transcribed using T7 RNA polymerase from a linearized plasmid containing the required sequence. The sequence is naturally found in Spinacia oleracea (Spinach) cholorplasts. Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using data obtained from homonuclear and heteronuclear experiments. |
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Sample preparation
Details | Contents: RNA molecule (1mM-2mM) in 50 mM NaCl, 2.5mM Cacodylate, 0.1mM EDTA, pH 6.0 Solvent system: H2O, D2O | |||||||||||||||
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics, molecular dynamics / Software ordinal: 1 Details: Distance, Dihedral, Planarity restraints were used in the first round, followed by dipolar couplings in the second round (See paper for details) | ||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 65 / Conformers submitted total number: 13 |